天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (2): 333-337.doi: 10.16333/j.1001-6880.2015.02.027

• 开发研究 • 上一篇    下一篇

超微粉碎对桑黄子实体粗多糖理化性质和刺激巨噬细胞活性的影响

李婷婷1,2,杨焱1,刘艳芳1*,邵倩1,3,张劲松1,鲍大鹏1   

  1. 1 农业部南方食用菌资源利用重点实验室 国家食用菌工程技术研究中心上海市农业遗传育种重点开放实验室 上海市农业科学院食用菌研究所,上海 201403;2 上海海洋大学食品学院;3 上海师范大学生命与环境科学学院,上海 200234
  • 出版日期:2015-02-15 发布日期:2015-02-15

Effects of Superfine Grinding on Physicochemical Properties and Macrophages activation Activity of Crude Polysaccharides from Phellinus baumii

LI Ting-ting1,2, YANG Yan1, LIU Yan-fang1*, SHAO Qian1,3, ZHANG Jing-song 1, BAO Da-peng 1   

  1. 1 National Engineering Research Center of Edible Fungi;Key Laboratory of Edible Fungi Resources and Utilization(South), Ministry of Agriculture,the People’s Republic of China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences, Shanghai 201403,China; 2 Shanghai Ocean University,Shanghai 201306,China; 3 Shanghai Normal University,Shanghai 200234,China
  • Online:2015-02-15 Published:2015-02-15

摘要: 以桑黄子实体为材料,比较了普通粉碎和超微粉碎对桑黄子实体多糖提取及其理化性质和刺激巨噬细胞活性的影响。结果表明,相对于传统粉碎方式,超微粉碎可以显著提高桑黄子实体多糖的得率,分级醇沉所得各组分中β-葡聚糖含量明显提高。HPSEC-MALLS-RI联用分析桑黄多糖分子量分布结果显示,超微粉碎所得各组分分子量较大,且分布范围较宽。两种方法处理所得的6个组分均具有刺激RAW264.7细胞释放NO的活性,其中30%乙醇沉淀组分(CW30和FP30)活性最好,超微粉碎后50%和70%乙醇沉淀组分(CW50和CW70)活性低于普通粉碎后所得的相应组分(FP50和FP70)。

关键词: 桑黄多糖, 超微粉碎, &beta, -葡聚糖, 分子量, 生物活性

Abstract: The physicochemical properties and macrophages activation activity of Phellinus baumii polysaccharides extracted from the fruiting bodies ground by different methods were studied in this paper.Compared with ordinary grinding methods,superfine grinding technology exerted significantly higher yields of crude polysaccharides,polysaccharide contents and β-glucan contents of six samples.HPSEC-MALLS-RI analysis showed the molecular weight distribution of P.baummii crude polysaccharides,which indicated superfine grinding technique generated higher molecular weight fractions and larger polydispersities.The 6 crude polysaccharide fractions had obvious effects on NO generation of RAW264.7 cells,which indicated that crude polysaccharides from P.baumii might have some immune-enhancing effects.Meanwhile,CW30 and FP30 showed the best immune-enhancing effects.However,CW50 and CW70 exerted lower immuneenhancing effects compared with FP50 and FP70.

Key words: Phellinus baumii polysaccharides, superfine grinding, &beta, -glucan, molecular weight, biological activity

中图分类号: 

R932