关键词: 樟属, 油樟, 悬浮细胞培养, 挥发性物质, 1, 8-桉叶油素, &gamma, -松油烯, &alpha, -松油醇

Abstract: Water extracts of 4 Cinnamomum plant leaves including Cinnamomum longepaniculatum N.Chao ex H.W.Li., Cinnamomum parthenoxylum, Cinnamomum camphora and Cinnamomum japonicum Sieb.were added to the cell suspension cultures of Cinnamomum longepaniculatum to study their effect on the cell growth and volatile metabolites accumulation.The results showed that the 4 targeted water extracts of Cinnamomum plant leaves had no effect on cell growth. However,leaf extracts had different effects on volatile metabolites accumulation in the cell suspension cultures of C.longepaniculatum. C.japonicum extract (4%) and C.camphora extract (1%) increased the total content of volatile metabolites in the cell suspension cultures of C.longepaniculatum by 78% and 45%, respectively.1% of C.japonicum extract was the optimal concentration which increased the accumulation of 1,8-cineole by 46%.Leaf extracts promoted the accumulation of γ-terpinene in the cell suspension cultures of C.longepaniculatum. 1% of  C.parthenoxylum, C.camphora and C.japonicum extracts increased the peak area of γ-terpinene from 0 pA to 0.39,0.27 and 0.32,respectively.4% of C.longepaniculatum extract increased the peak area of γ-terpinene from 0 pA to 0.27 pA. 0.25% of C.japonicum and C.camphora extracts had the strongest promotion effects for α-terpineol.The content of α- terpineol was 1.62 and 1.24 times as blank. This study revealed that leaf extracts from Cinnamomum plant leaves affected the accumulation of volatile metabolites in the cell suspension cultures of C.longepaniculatum, which laid a foundation for further study.

Key words: Cinnamomum plants, Cinnamomum longepaniculatum N.Chao ex H.W.Li.;suspension cell culture;volatile products;1,8-cineole;γ-terpinene;α-terpineol