天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (8): 1340-1345.doi: 10.16333/j.1001-6880.2015.08.004

• 研究论文 • 上一篇    下一篇

核桃楸皮多糖的分离纯化及抗癌活性研究

梁启超1*,邹桂华1,刘爽2,沈广志1,吴宜艳1   

  1. 1 牡丹江医学院药学院;2牡丹江市心血管病医院,牡丹江 157011
  • 出版日期:2015-08-29 发布日期:2015-09-01

Separation,Purification and Anticancer Activities of Polysaccharides from the Bark of Juglans mandshurica

LIANG Qi-chao1*,ZOU Gui-hua1,LIU Shuang2,SHEN Guang-zhi1, WU Yi-yan1   

  1. 1 College of Pharmacy,Mudanjiang Medical College; 2 Department of Pharmacy,Mudanjiang Cardiovascular Hospital,Heilongjiang Mudanjiang 157011,China
  • Online:2015-08-29 Published:2015-09-01

摘要: 采用水提醇沉法提取核桃楸皮多糖(JMPS),并利用色谱法对其进一步纯化得到四种均一多糖JMPS-1、JMPS-2a、JMPS-2b和JMPS-3,其分子量分别为227.9 kDa、28.6 kDa、23.1 kDa和12.7 kDa。使用气相色谱法测定组分的单糖组成;利用红外光谱研究多糖组分的结构特征。体外抗肿瘤实验表明JMPS-1对SMMC-7721细胞具有较强抑制作用,流式细胞实验发现JMPS-1将SMMC-7721细胞的阻滞在G2/M期和S期。JMPS-1具有抗癌活性,可用作天然的抗癌物质。

关键词: 核桃楸皮, 多糖, 理化性质, 抗癌

Abstract: The bark of Juglans mandshurica polysaccharides (JMPS) were obtained by hot water extraction,ethanol precipitation,then further purified by column chromatography to afford four homogeneous polysaccharides,namely JMPS-1, JMPS-2a,JMPS-2b and JMPS-3,with average molecular weights approximately of 227.9 kDa,28.6 kDa,23.1 kDa,and 12.7 kDa,respectively.The monosaccharide compositions of polysaccharides were analyzed by GC-FID.The structural characteristics of JMPS fractions were determined by IR spectrophotometer.The evaluation of anticancer activity suggested that JMPS-1 had strong inhibition activities against SMMC-7721 cells.Flow cytometry analysis showed that JMPS-1 can induce cell cycle arrest at G2/M and S phase in SMMC-7721 cell.JMPS-1 had demonstrated anticancer activities and may be explored as a natural anticancer substance.

Key words: Juglans mandshurica, polysaccharides;characterization;anticancer activity

中图分类号: 

R285.5