天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (10): 1759-1764.doi: 10.16333/j.1001-6880.2015.10.014

• 研究简报 • 上一篇    下一篇

响应面法优化金刷把多糖提取工艺

刘婷,尚姣,王慧,郑娟娟,赵洁,房敏峰*   

  1. 西部资源生物与现代生物技术教育部重点实验室 西北大学生命科学学院,西安 710069
  • 出版日期:2015-10-30 发布日期:2015-10-30

Optimization of Extraction of Polysaccharide from Lethariella cladonioides Using Response Surface Methodology

LIU Ting, SHANG Jiao, WANG Hui, ZHENG Juan-juan, ZHAO Jie, FANG Min-feng*   

  1. Key Laboratory of Resources Biology and Biotechnology in Western China,Ministry of Education The College of Life Sciences,Northwest University,Xi’an 710069,China
  • Online:2015-10-30 Published:2015-10-30

摘要: 利用响应面法优化金刷把多糖的提取工艺。在单因素试验的基础上,选择提取温度、提取时间、料液比为自变量,以多糖提取率为响应值,进行Box-Benhnken中心组合实验设计,应用响应曲面分析方法优化提取条件,得到金刷把多糖最佳提取工艺条件如下:提取温度95 ℃,提取时间2.5 h,料液比1:20 g/mL,此时金刷把多糖提取率的理论预测值为4.62%,最优条件下多糖得率的实验值为4.42%,与理论值的相对误差为4.3%。经过响应面法优化提取工艺,提高了提取率,适用于金刷把粗多糖的提取。

关键词: 金刷把, 地衣多糖, 响应面法

Abstract: The objective of this study was to optimize the extraction of polysaccharide from Lethariella cladonioides (NyL.) Krog through response surface methodology.Based on single factor experiments,extraction temperature,extraction duration,solid-liquid ratio were selected for Box-Behnken central composite design.By means of regression analysis,the results showed that the optimal conditions to extract polysaccharide from L.cladonioides were as follows:extraction temperature of 95 ℃,extraction duration of 2.5 h,solid-liquid ratio of 20 mL/g.Under these conditions,the extraction yield of polysaccharide reached 4.42%.Compared with the theoretical value (4.62%),the relative error was 4.3%.The results showed that the optimized process improved extraction efficiency.It can be used in practical forecasting.

Key words: Lethariella cladonioides (NyL.) Krog, Lichen polysaccharide, response surface methodology

中图分类号: 

TS201