天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (Suppl): 135-139.doi: 10.16333/j.1001-6880.2015.S.033

• 开发研究 • 上一篇    下一篇

亳芍多酚提取工艺及栽培年限对总多酚积累的影响研究

陈乃东1,2,3*,陈瀚1,朱旺生1,2,于丹1   

  1. 1 皖西学院生物与制药工程学院;2 皖西中药与天然药物工程技术研究中心,六安 237012;3安徽医科大学,合肥 230032
  • 出版日期:2015-12-01 发布日期:2016-01-12

Extraction and Accumulation of Polyphenol from Bozhou Peony Paeonia lactiflora with different plant ages

CHEN Nai-dong1,2,3*,CHEN Han1,ZHU Wang-sheng1,YU Dan1   

  1. 1 College of Biotechnology and Pharmaceutical Engineering,West Anhui University; 2 West Anhui Biotechnology Research Center of Natural Medicine and Traditional  Chinese Medicine,West Anhui University,Lu’an 237012,China; 3 Anhui Medical University,Hefei 230032,China
  • Online:2015-12-01 Published:2016-01-12

摘要: 以没食子酸为对照品,采用Folin-Ciocaltcu比色法、在正交实验确定亳芍总多酚最佳提取条件基础上对不同栽培年限的亳芍多酚含量进行测定。结果表明,亳芍总多酚最佳提取工艺条件为:乙醇浓度为60%,料液比为 1:35 g/mL,提取时间为2.0 h,提取温度为 70 ℃。在此条件下测得栽培1~7的亳芍总多酚占其干重的含量依次为:2.63±0.49%、3.82±0.55%、3.64±0.64%、2.42±0.61%、2.51±0.76%、2.57±0.87%、2.55±0.72%。研究结果为亳芍的规范化栽培和临床用药提供参考依据。

关键词: 亳芍, 总多酚, 规范化栽培

Abstract: In order to explore the total polyphenol contents of different years-old Bozhou Peony and provide references for its standard cultivation and medical application,the Folin-Ciocaltcu chromatometry with gallic acid as standard preparation was applied to detect the contents of polyphenol from different years-old Bozhou peony on the optional conditions which was obtained from the orthogonal experiments.The results showed that most of the polyphenol could be extracted from Bozhou Peony using 60% ethanol as extraction solvent with ratio of material to liquid 1:35 on the condition that extraction time and temperature were 2.0 h and 70 ℃,respectively.Remarkable variation of the polyphenol content was existed between different years-old Bozhou Peonys with the orders of cultivation years 17:2.63±0.49%,3.82±0.55%,3.64±0.64%,2.42±0.61%,2.51±0.76%,2.57±0.87%,2.55±0.72%.Our experiments might provide valuable references for the standardized cultivation and clinical medication of Bozhou Peony.

Key words: Bozhou Peony Paeonia lactiflora, total polyphenols, standardized cultivation

中图分类号: 

R284.2