关键词: 球毛壳菌, 次生代谢产物, 分离纯化, 免疫活性

Abstract: Ten compounds were isolated and purified from the metabolites of Chaetomium globosum CIB-160 by silica gel column chromatography,HPLC,Sephadex LH-20 chromatography.Their chemical structures were identified as chaetoglobosins A (1),C (2),E (3),F (5),Fex (6),W (7),penochalasin F (4),5-(methyl-2butenyl)-indole-2,3-dione (8),chaetoviridin A (9),and cochliodone A (10) on the basis of NMR,HR-MS and optical rotation data,compound 8 and 10 were first reported from wild strain of C.globosum. Compounds 1-6 showed significant inhibition of mouse spleen cell proliferation,their IC₅₀ values were 0.21,2.8,2.3,2.2,1.7,2.7 μM,respectively.The IC₅₀ values of mice spleen cell survival rates successively were 0.82,7.5,2.3,6.1,4.6,6.7 μM,which indicated compounds 1-6 possessed strong cytotoxicity.

Key words: Chaetomium globosum, secondary metabolites, isolation and purification, immunological activity