天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (4): 542-546.doi: 10.16333/j.1001-6880.2016.4.013

• 研究简报 • 上一篇    下一篇

不同种源的太子参多糖含量及其单糖组成GC-MS研究

夏和先1,2,3，陈乃东1,2*，姚厚军1,2，王海军1，柯成1，孙叶超1   

  1. 1 皖西学院生物与制药工程学院，六安 237012；2 皖西中药与天然药物工程技术研究中心，六安 237012；3 皖西卫生职业学院，六安 237005
  • 出版日期:2016-04-30 发布日期:2016-05-13

Contents and Mono-saccharide Compositions of Polysaccharides from Different Origins of Psudostellaria heterophylla

XIA He-xian1,2,3,CHEN Nai-dong 1,2*,YAO Hou-jun1,2,WANG Hai-jun1,KE Cheng3,SUN Ye-chao3   

  1. 1 College of Biotechnology and Pharmaceutical Engineering,West Anhui University,Lu’an 237012,China; 2 West Anhui Biotechnology Research Center of Natural Medicine and Traditional Chinese Medicine, West Anhui University,Lu’an 237012,China;3West Anhui Health Vocational College,Lu’an 237005,China
  • Online:2016-04-30 Published:2016-05-13

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摘要： 研究不同种源太子参多糖含量以及单糖组成差异，为太子参质量评价和临床用药提供参考依据，采用水提-醇沉法提取太子参多糖，以葡萄糖为标品，蒽酮-硫酸比色法测定多糖含量。盐酸甲醇水解、三甲基硅烷（TMS）柱前衍生、气-质联用法（GC-MS）分析不同种源太子参多糖的单糖组成。结果表明，组培太子参与野生太子参多糖的含量分别为19.05±1.38%、22.63±2.18%；不同种源太子参多糖均由葡萄糖、果糖、甘露糖、半乳糖、半乳糖醛酸、阿拉伯糖、鼠李糖七种单糖组成，但单糖的比例明显不同。如以多糖含量为标准，野生太子参多糖含量高于组培太子参，品质更佳；多糖中单糖比例不同，提示不同种源的太子参多糖结构可能存在不同。

关键词: 太子参, 多糖, 单糖组成, 气-质联用, 种源

Abstract: In order to provide references for the quality evaluation and clinical medication of Psudostellaria heterophylla,the contents and monosaccharide compositions of polysaccharide from tissue-cultured P.heterophylla and its wild correspondence were investigated.The polysaccharides were extracted from P.heterophylla by a water-extraction and alcohol-precipitation method and the contents were determined by anthrone-sulfuric acid colorimetric method.The obtained polysaccharides were sequentially treated with 0.5 M HCl-methanol,trimethyl-silane derivatization and GC-MS analysis was carried out to study its monosaccharide compositions.The results showed that the contents of polysaccharide in tissue-cultured and wild P.heterophylla were 19.05±1.38% and 22.63±2.18%,respectively.GC-MS analysis revealed that polysaccharide in tissue-cultured and wild P.heterophylla were both consisted of glucose,fructose,mannose,galactose,galacturonic acid,arabinose and rhamnose,but the proportion of monosaccharides varied remarkably in the polysaccharides from different origins.

Key words: Psudostellaria heterophylla, polysaccharide, monosaccharide composition, GC-MS, origin

中图分类号: 

R283.3

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