关键词: 氧化槐定碱, 药代动力学, 高效液相色谱法, 大鼠

Abstract: To establish a method for the determination of oxysophoridine in rats plasma by HPLC-UV and to explore its profiles of pharmacokinetics.Plasma samples were extracted with methanol and separated on a ZORBAX XDB-C₁₈ (150 mm × 4.6 mm,4 μm) column with a mobile phase consisting of phosphate buffer (pH 3.00) and acetonitrile (96:4,v/v).The detection wavelength was 210 nm.The assay was linear in the range of 2.50~500.00 μg/mL (r=0.9974).The recovery of oxysophoridine from plasma was 77.3% to 89.9%,and RSDs of interday and intraday were less than 10%.After intravenous injection of oxysophoridine (50,100 mg/kg) to rats,its t_1/2, AUC and C_max were (37.24±9.27) and (34.25±8.82) min,(1998.76±178.35) and (5130.62±1450.86) mg/L/min,and (89.53±10.47) and (247.35±112.02) mg/L,respectively.The method was convenient and economical.It was suitable for the study on pharmacokinetics of oxysophoridine in vivo.

Key words: oxysophoridine, pharmacokinetics, HPLC-UV, rat