泽泻汤加味方对高脂血症大鼠AQP3基因蛋白表达的影响

doi:10.16333/j.1001-6880.2017.10.007

天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (10): 1671-1676.doi: 10.16333/j.1001-6880.2017.10.007

泽泻汤加味方对高脂血症大鼠AQP3基因蛋白表达的影响

王亚芬1，韩雪2，张一昕2*，段思明2，郝蕾2，王茜2，刘晨旭2

¹河北医科大学，石家庄 050017；²河北中医学院，石家庄 050200

出版日期:2017-10-27 发布日期:2017-10-27
基金资助:
河北省中医药管理局科学技术研究计划（201 50030）；河北省高等学校科学技术研究项目（QN2015251）

Effects of Modified Rhizoma Alismatis Decoction on Expression of AQP3 in Rats with Hyperlipidemia

WANG Ya-fen1, HAN Xue2, ZHANG Yi-xin2*, DUAN Si-ming2, HAO Lei2, WANG Xi2, LIU Chen-xu2

¹Hebei Medical University,Shijiazhuang 050017, China; ²Hebei University of Traditional Chinese Medicine,Shijiazhuang 050200, China

Online:2017-10-27 Published:2017-10-27

摘要/Abstract

摘要： 通过观察泽泻汤加味方对高脂血症大鼠血脂以及结肠组织水通道蛋白3（AQP3）表达的影响，探讨其作用机制。将60只雄性SD大鼠随机分为空白组、模型组、辛伐他汀对照组、泽泻汤加味方高、中、低剂量组，采用喂饲高脂饲料方法复制动物模型，造模的同时给予相应药物治疗。用药5周以后，观察大鼠血清中TG、TC、HDL-C、LDL-C含量的变化，运用RT-PCR方法检测了大鼠结肠组织AQP3基因表达情况，运用免疫组化和Western Blot方法检测了大鼠结肠组织AQP3蛋白的表达情况。结果显示，模型组大鼠血脂中TG、TC、LDL-C的含量及结肠中AQP3基因蛋白的表达明显升高，HDL-C含量显著降低。各治疗组大鼠血脂中TG、TC、LDL-C的含量及结肠组织AQP3基因蛋白的表达均降低，HDL-C含量升高。提示泽泻汤加味方对高脂血症大鼠血脂具有良好的调节作用，其通过上调结肠组织AQP3基因、蛋白的表达，以调节水液代谢，阻止痰湿的生成，祛除致病因素，可能是其治疗高脂血症的分子机制之一。

关键词: 泽泻汤加味方, 高脂血症, 水通道蛋白3, 实验研究

Abstract: To discuss the mechanism of modified Rhizoma Alismatis Decoction on prevention and treatment of hyperlipemia,through investigating hepatic expressions of AQP3 in rats with hyperlipemia.60 Male Sprague-Dawley rats were randomly divided into 6 groups:Control group,Model control group,Simvastatin control group,High-dose of modified Rhizoma Alismatis Decoction group,Middle-dose of modified Rhizoma Alismatis Decoction group,Low-dose of modified Rhizoma Alismatis Decoction group.Rats in control group were fed with ordinary forage,and the others were fed with high-fat forage.At the same time,each treatment group was administrated with appropriate drug.Then TC,TG,HDL-C and LDL-C of serum were detected,the genetic expression of AQP3 in colon tissue were detected with RT-PCR and the protein expression of AQP3 in colon tissue were detected with immunohistochemical means and Western Blot after 5 weeks.In the model control group,the content of TG,TC,LDL-C in serum and the expression of AQP3 in colon tissue were significantly elevated,while the content of HDL-C was significantly reduced.After treatment,the contents of TG,TC,LDL-C and the expression of AQP3 in every treatment groups were reduced,while the contents of HDL-C were elevated.Modified Rhizoma Alismatis decoction had a satisfying effect on regulating the blood lipid.Increasing the genetic and protein expression of AQP3 in colon tissue,regulating the water fluid metabolism,preventing the formation of sputum and removing the pathogenic factors could be viewed as mechanisms of modified Rhizoma Alismatis Decoction concerning prevention and treatment of hyperlipemia.

Key words: modified Rhizoma Alismatis decoction, hyperlipidemia, AQP3, experimental research

中图分类号:

R285.5

王亚芬1，韩雪2，张一昕2*，段思明2，郝蕾2，王茜2，刘晨旭2. 泽泻汤加味方对高脂血症大鼠AQP3基因蛋白表达的影响[J]. 天然产物研究与开发, 2017, 29(10): 1671-1676.

WANG Ya-fen1, HAN Xue2, ZHANG Yi-xin2*, DUAN Si-ming2, HAO Lei2, WANG Xi2, LIU Chen-xu2. Effects of Modified Rhizoma Alismatis Decoction on Expression of AQP3 in Rats with Hyperlipidemia[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2017, 29(10): 1671-1676.

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泽泻汤加味方对高脂血症大鼠AQP3基因蛋白表达的影响

Effects of Modified Rhizoma Alismatis Decoction on Expression of AQP3 in Rats with Hyperlipidemia

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