天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (10): 1745-1751.doi: 10.16333/j.1001-6880.2017.10.019

• 开发研究 • 上一篇    下一篇

鱼腥草叶多糖的抗氧化性及抑菌特性

黎海梅,杜阳敏,陈俊,周华*   

  1. 暨南大学理工学院食品科学与工程系,广州 510632
  • 出版日期:2017-10-27 发布日期:2017-10-27
  • 基金资助:

    国家自然科学资金(31101323)

Antioxidant and Antimicrobial Properties of Polysaccharide from Houttuynia cordata Leaf

LI Hai-mei, DU Yang-min, CHEN Jun, ZHOU Hua*   

  1. Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China
  • Online:2017-10-27 Published:2017-10-27

摘要: 采用Toyopearl柱层析将鱼腥草叶多糖(Houttuynia cordata Leaf Polysaccharides,HCP)进行分离、纯化,用GC-MS色谱分析多糖的单糖组成及比例。研究鱼腥草叶多糖的体外抗氧化能力(DPPH·的清除能力、对Fe2+的鳌合能力、铁氰化钾的还原能力),测试了它们的抑菌效果及最低抑制浓度。结果表明,3种多糖均对DPPH·具有一定的清除能力、对Fe2+有鳌合作用以及对铁氰化钾有一定的还原效果,其作用强度为HCP1>HCP2>HCP3;抑菌实验表明鱼腥草叶多糖对多种供试菌均有一定的抑制效果,其中HCP1、HCP2对金黄色葡萄球菌的抑制效果最强,MIC为0.9375 mg/mL,HCP3对金黄色葡萄球菌和大肠杆菌的抑制效果最强,MIC为1.875 mg/mL。

关键词: 鱼腥草叶, 多糖, 抗氧化, 抑菌

Abstract: Houttuynia cordata Leaf Polysaccharides (HCP) was isolated and purified by Toyopearl column chromatography.The composition and proportion of polysaccharides were analyzed by GC-MS.The in vitro antioxidant capacity (DPPH scavenging ability,chelating ability of Fe2+ and the reducing ability of potassium ferricyanide) were studied.The antibacterial effect and the minimum inhibitory concentration were tested.The results showed that polysaccharides (HCP1,HCP2,HCP3) had certain scavenging ability to DPPH,the chelating effect on Fe2+ and the reduction effect of potassium ferricyanide,and the effect intensity was HCP1> HCP2> HCP3;the bacteriostatic experiment showed that the inhibitory effect of HCP1 and HCP2 on Staphylococcus aureus was the strongest with MIC of 0.9375 mg/mL,and the inhibitory effect of HCP3 on S.aureus and E.coli was the most effective with MIC of 1.875 mg/mL.

Key words: Houttuynia cordata Leaf, polysaccharide, antioxidant, antibacterial

中图分类号: 

O629.12