天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (10): 1773-1778.doi: 10.16333/j.1001-6880.2017.10.023

• 开发研究 • 上一篇    下一篇

响应面法优化黑根霉胞外多糖脱蛋白工艺研究

秦国正1,2,3,琚勇1,张明芮1,李萍1,2,3,柳春燕2,3,唐保露1,薛信磊1,王国栋1,2,3*   

  1. 1皖南医学院药学院;2安徽省多糖药物工程技术研究中心;3活性生物大分子研究安徽省重点实验室,芜湖 241002
  • 出版日期:2017-10-27 发布日期:2017-10-27
  • 基金资助:

    安徽高校自然科学研究项目(KJ2016A723,KJ2015A1 99,KJ2017A257);安徽省自然科学基金(1508085M H191);皖南医学院中青年科研基金(WK201517,WK201601,WK201610);大学生创新创业训练计划(201510368017,201510368119,201610368120)

Optimization of Deproteinization Process of Exopolysaccharides from Rhizopusnigricans by Response Surface Methodology

QIN Guo-zheng1,2,3, JU Yong1, ZHANG Ming-rui1, LI Ping1,2,3, LIU Chun-yan2,3, TANG Bao-lu1, XUE Xin-lei1, WANG Guo-dong1,2,3*   

  1. 1School of Pharmacy, Wannan Medical College; 2Anhui Provincial Engineering Research Center for Polysaccharide Drugs; 3Anhui Province Key Laboratory of Active Biological Macro-molecules, Wuhu 241002, China
  • Online:2017-10-27 Published:2017-10-27

摘要: 采用单因素实验结合Box-Behnken中心组合设计方法,以蛋白质脱除率和综合指数为指标,考察木瓜蛋白酶浓度、酶解温度和酶解时间对黑根霉胞外多糖酶-Sevag法脱蛋白工艺的影响。经过响应面实验优化,确定最优条件为木瓜蛋白酶浓度425 U/mL、酶解温度62 ℃、酶解时间90 min,经三次重复实验验证,蛋白质脱除率与综合指数均值分别为54.08%和2.07。

关键词: 黑根霉, 胞外多糖, 脱蛋白, 酶-Sevag法, 响应面法

Abstract: The effects of papain concentration,enzymolysis temperature and time on the deproteinization of extracellular polysaccharide from Rhizopusnigricans  in Enzyme-Sevag process were investigated by single-factor experiment combining with central composite design method of Box-Behnken.The optimal process parameters were determined based on the deproteinization rate and comprehensive index.After optimizing by response surface methodology,the optimal process parameters were confirmed as follows:papain concentration of 425 U/mL,enzymolysis temperature of 62  ℃ and time of 90 min.Following repeated experiments three times,the average deproteinization rateand comprehensive index were determined as 54.08 % and 2.07,respectively.

Key words: Rhizopusnigricans, exopolysaccharides, deproteinization, enzyme-sevag process, response surface methodology

中图分类号: 

R284.2