关键词: 绿原酸提取物, 抑菌效果, 抗氧化作用

Abstract: The aim of this study was to study antimicrobial and antioxidant effects of chlorogenic acid extracts from Gynuraprocumbens,Lonicera japonica Thunb. and Eucommiaulmoides leaves,and to provide a reference for its usage as a natural food preservative and antioxidant.The antibacterial effects were studied using disc diffusion method against several common pathogens.The antioxidant effect of chlorogenic acid extractsfrom G.procumbens,L.japonica and E.ulmoides leaves,including anti-lipid peroxidation,reducing capacity,DPPH scavenging ability were studied respectively.The inhibition of chlorogenic acid extracts on bacteria were very strong,especially on Staphylococcus aureus,at the concentration of 100 mg/mL,the bacteriostatic ring were up to 21.4 mm,23.6 mm,24.7mm,respectively for chlorogenic acidextracts from the three species.At the same time,it had obvious inhibitory effects on E.coli  and S.bacteria,but the inhibitory effect on the yeast was not obvious.The strength and the sequence of antibacterial compounds were: L.japonica chlorogenic acid extract, E.ulmoides chlorogenic acid extract, G.procumbens chlorogenic acid extract.The anti-lipid peroxidation ability,reducing power and DPPH scavenging ability of L.japonicachlorogenic acidextract were higher than those of the other two extracts. L.japonica chlorogenic acid extract had a stronger antibacterial activity and antioxidant effect among three chlorogenic acidextracts.

Key words: chlorogenic acid extracts, antibacterial effect, antioxidant effect