关键词: 配位体交换色谱, 单糖, 水解液, 废渣, 槐米

Abstract: In recent years,conversion of biomass materials to monosaccharides has attracted significant attention. In order to develop testing techniques for efficient usage of Flos Sophorae residue,the determination method of 4 monosaccharides in hydrolysate from Flos Sophorae residue was established with lead(Pb²⁺) ligand exchange high performance liquid chromatography(LEX-HPLC) and ion chromatography was used to verify the feasibility of this method. Influence of separation by different counterions(Pb²⁺ & Ca²⁺) in column was investigated. The lead(Pb²⁺) ligand exchange was applied in the actual samples detection finally. A Sepax Carbomix Pb-NP5(300×7.8 mm ID,5 μm,8% crosslinkage) column was used for the separation with ultrapure water as mobile phase. The flow rate of mobile phase was 0.50 mL/min at a column temperature of 75 ℃. Temperature of refractive index detector(RID) was 30 ℃. Samples were pretreated by solid phase extraction(SPE) with activated carbon to remove colorings. The results showed that the separation was achieved within 25 min. The calibration curve was linear in the mass concentration range of 0.2-20.0 mg/mL,the limit of detection(LOD) of glucose,xylose,galactose and arabinose were 1.94-1.98 μg/mL,respectively. Content of arabinose in samples exceeded 67% and it had a high usage value. The method was simple,accurate,economical and reproducible.

Key words: LEX-HPLC, monosaccharide, hydrolysate, residue, flos sophorae