天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (7): 1182-1187.doi: 10.16333/j.1001-6880.2017.7.018

• 开发研究 • 上一篇    下一篇

六妹羊肚菌多糖部分理化性质及抗氧化活性研究

熊川1，罗强2，李强1，陈诚3，陈祖琴1，黄文丽1*   

  1. 1 四川省农业科学院生物技术核技术研究所，成都 610061；2 重庆医科大学附属第二医院 感染病分子重点实验室 肝炎研究所，重庆 400010；3四川省农业科学院植物保护研究所，成都 610066
  • 出版日期:2017-07-29 发布日期:2017-08-11

Physical and Chemical Properties of Polysaccharide Isolated from the Fruiting Bodies of Morchella sextelata and its Antioxidant Effect

XIONG Chuan1,LUO Qiang2,LI Qiang1,CHEN Cheng3,CHEN Zu-qin1,HUANG Wen-li1*   

  1. 1 Biotechnology and Nuclear Technology Research Institute,Sichuan Academy of Agricultural  Sciences ,Chengdu 610061,China;2Key Laboratory of Molecular Biology for Infectious  Diseases (Ministry of Education),Institutefor Viral Hepatitis,Department of Infectious Diseases, The Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010,China;3Institute of Plant Protection,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China
  • Online:2017-07-29 Published:2017-08-11

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摘要： 分离纯化人工栽培的六妹羊肚菌子实体多糖，对其结构和抗氧化活性进行研究。采用水提醇沉法提取六妹羊肚菌子实体多糖（MSP），采用DE-52纤维素柱和Sephadex G-100进行多糖的分离纯化，借助HPGPC和HPLC测定多糖分子量及单糖组成。通过DPPH自由基、羟自由基和超氧阴离子自由基的清除作用，评估其体外抗氧化活性。构建H2O2介导的氧化压力损伤的PC12细胞模型，评估其基于抗氧化的神经保护作用。结果显示，六妹羊肚菌水溶性多糖平均分子量为287 588 Da，单糖组成为甘露糖，葡萄糖和半乳糖，占比约为9:1:6。六妹羊肚菌多糖具有优良的自由基清除活性，同时，能够通过重塑SOD、CAT、GSH-Px等抗氧化酶系活性，缓解H2O2诱发的氧化压力的细胞损伤，抑制PC12细胞凋亡。涉及通路为Bax/Bcl及Caspase。六妹羊肚菌水溶性多糖具有优良的抗氧化活性，表现出一定的神经保护作用。

关键词: 六妹羊肚菌, 多糖提取, PC12细胞, 抗氧化, 神经保护

Abstract: This article mainly studied the extraction,purification,structure and antioxidant effects of a polysaccharide (MSP) purified from the fruiting bodies of Morchella sextelata M.Kuo.The polysaccharides of M.sextelata were extracted with boiling water and purified by DE-52 column and Sephadex G-100 column.The molecular weight,monosaccharide composition were analyzed by HPGPC and HPLC.In order to investigate the neuroprotective role of MSP,the antioxidant activity of MSP against the H2O2-induced cytotoxicity and the underlying preventative mechanisms in rat pheochromocytoma (PC12) cells were illustrated.It was shown that the molecular weight of MSP was 287 588 Da(287 588 Da),the monosaccharide composition of MSPwasmainly composed of mannose,glucose and galactose,with a ratio near 9:1:6.MSP can considerably enhance the viability of PC12 cells exposure to H2O2 and increase the activities of antioxidant enzyme like SOD,CAT and GSH-Px.It inhibited cell apoptosis via reducing caspase-3 activation and down-regulation Bax/Bcl-2 ratio.Accordingly,MSP can be used as a promising neuroprotective compound for nervous diseases treatment.

Key words: Morchella sextelata, extraction of polysaccharide, PC-12 cell, antioxidant, neuroprotective

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