关键词: 二烯丙基二硫, IL-1&beta, SD大鼠关节软骨细胞, 双向调节

Abstract: To explore the effect of diallyl disulfide (DADS) on articular cartilage cell induced by IL-1β  in SD rats.The growth inhibition of articular cartilage cell induced by IL-1β in SD rats following DADS treatment was measured by CCK8 assay and plate colony formation assay; The protein expression of MMP13,TIMP1 and Collagen II were determined using Western blot.DADS (12.5 and 25 μmol/L) significantly increased the cell proliferation and high colony formation capacity; DADS (50 and 100 μmol/L) significantly suppressed the cell proliferation and lowered colony formation capacity (P<0.05); Western blot demonstrated DADS markedly down regulated the expression of MMP13,and increased the expression of Collagen II and TIMP1 in the cells.These results indicated that DADS had two-way regulation effect on SD rat articular cartilage cell induced by IL-1β, DADS (25 μmol/L) had a protective effect on SD rat articular cartilage cell induced by IL-1β, DADS (50 and 100 μmol/L) can inhibit articular cartilage cell by IL-1β in SD rats,the protection molecular mechanism was possibly related to downregulation of the expression of MMP13 by DADS,and increased the expression of Collagen II and TIMP1 on SD rat articular cartilage cell with osteoarthritis.

Key words: diallyl disulfide, IL-1β, articular cartilage cell, two-way regulation