天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (增刊1): 158-165.doi: 10.16333/j.1001-6880.2017.S.032

• 开发研究 • 上一篇    下一篇

无子刺梨总黄酮的超声辅助提取优化及其抗氧化活性

但汉龙1,2，李娜1，罗旭璐1，刘云1，张德国2，阚欢1，李永和2，赵平1*   

  1. 1 西南林业大学 西南山地森林资源保育与利用省部共建教育部重点实验室；2西南林业大学 云南生物多样性研究院，昆明 650224
  • 出版日期:2017-08-02 发布日期:2017-08-02

Optimization of Ultrasound-assisted Extraction and Antioxidant Activities of Total Flavonoids from the Fruits of Rosa sterilis

DAN Han-long1,2,LI Na1,LUO Xu-lu1,LIU Yun1,ZHANG De-guo2,KAN Huan1,LI Yong-he2,ZHAO Ping1*   

  1. 1Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education,Southwest Forestry University;2 Yunnan Academy of  Biodiversity,Southwest Forestry University,Kunming 650224,China
  • Online:2017-08-02 Published:2017-08-02

PDF (PC)

283

摘要： 采用超声辅助乙醇法从无子刺梨果实中提取黄酮，通过单因素试验考察了提取时间、料液比、乙醇体积分数和提取次数4个因素对无子刺梨黄酮得率的影响，并采用响应面法确定无子刺梨黄酮的最佳工艺条件为：提取时间70 min、料液比1:22.39、乙醇体积分数52.2%、提取次数4次，在此优化条件下无子刺梨黄酮的理论提取率可达到0.828%。依实际试验情况，将其修正为提取时间70 min、料液比1:23、乙醇体积分数52%、提取次数4次，经验证此条件下无子刺梨黄酮的提取率为0.817%，与理论值较为接近，表明采用响应面法分析优化无子刺梨黄酮超声辅助提取工艺的方法可行。以Vc为阳性对照，采用DPPH和ABTS自由基清除活性测试方法对该工艺制备所得无子刺梨总黄酮的抗氧化活性进行了测定。无子刺梨总黄酮对DPPH和ABTS自由基的半清除浓度（SC50）分别为6.75 ± 0.54 μg/mL和11.43 ± 0.17 μg/mL，表明其具有明显优于Vc的抗氧化活性。

关键词: 无子刺梨, 黄酮, 超声辅助提取, 响应面法, 抗氧化活性

Abstract: The ultrasoundassisted extraction of total flavonoids from the fruits of Rosa sterilis was carried out.The effects of extraction time,materialliquid ratio,ethanol concentration and extraction number on the extraction rate of total flavonoids were investigated by single factor experiment,and the extraction process were optimized using response surface methodology.The results showed that the optimal extraction conditions were 70 min of extraction time,1:22.39 of materialliquid ratio,52.2% of ethanol concentration,and 4 times of extraction number.Under this optimal condition,the theoretic extraction rate of total flavonoids could reach to 0.828%.According to the actual experiment,the optimum parameters were modified as:extraction time 70 min,materialliquid ratio 1:23,ethanol concentration 52% and extraction number 4 times,and the actual extraction rate of total flavonoids was 0.817% that is close to the theoretic value.The results showed that the response surface method to optimize the ultrasonicassisted extraction of total flavonoids from the fruits of R.sterilis is feasible.Using Vc as a positive control,the antioxidant activities of total flavonoids obtained from above optimal condition were determined by DPPH and ABTS free radical scavenging assays.The SC50 values of total flavonoids against DPPH and ABTS free radicals were 6.75±0.54 μg/mL and 11.43±0.17 μg/mL respectively indicated that the total flavonoids from R.sterilis possesses a significant antioxidant activity than Vc.

Key words: Rosa sterilis, flavonoids, ultrasonicassisted extraction, response surface methodology, antioxidant activity

版权所有 © 《天然产物研究与开发》编辑部
地址：四川天府新区群贤南街289号　邮编：610299
电话：028-85210304　QQ群：342035423　Email：trcw@clas.ac.cn　
本系统由北京玛格泰克科技发展有限公司设计开发
总访问量：　今日访问：　在线人数：