关键词: 锁阳, 原花青素, 微波法, 抗糖基化

Abstract: Proanthocyanidins obtained by different extraction methods were researched on the yield,antioxidant and antiglycation activities.Proanthocyanidins from Cynomorium songaricum Rupr.was extracted by ultrasound-assisted extraction (UAE),ethanol-ammonium sulfate two-phase extraction,water extraction,microwave-assisted extraction (MAE),and enzymatic hydrolysis,respectively.The content was determined by Vanillin-HCl method.DPPH· scavenge activity and hydroxyl radical assay were conducted to evaluate the antioxidant abilities of proanthocyanidins prepared by different methods.Bovine serum albumin (BSA)-Glucose and BSA-methyglyoxal (MGO) models were applied to the measurement of antiglycation capacity.The results indicated that MAE resulted in higher yields,15.00% and a shorter extraction time of 19 min.In terms of DPPH radical scavenging activities,MAE showed the best antioxidant capacity,followed by enzymatic hydrolysis,UAE,ethanol-ammonium sulfate two-phase extraction,water extraction.MAE showed the best the scavenging rate of OH,followed by enzymatic hydrolysis,UAE,water extraction,and ethanol-ammonium sulfate two-phase extraction.The proanthocyanidins prepared by MAE method had excellent antiglycation activity in BSA-glucose and BSA-MGO model,respectively.Antioxidant showed no correlation with antiglycation activity.

Key words: Cynomorium songaricum Rupr., proanthocyanidins, microwave-assisted extraction, antiglycation