关键词: 云南枸杞, 根部, 酰胺类, 抗炎活性

Abstract: The amide constituents in the ethanol extract of the root of Lycium yunnanense Kuang were isolated and purified by column chromatography on silica gel,Sephadex LH-20 as well as preparative HPLC.Their structures were identified by physical and spectral data.Fifteen amides were isolated and elucidated as N-trans-feruloyltyramine (1),N-cis-feruloyltyramine (2),dihydro-N-trans-feruloyltyramine(3),3-(4-hydroxy-3-methoxyphenyl)-N-［2-(4-hydroxyphenyl)-2-methoxyethyl］acrylamide (4),N-trans-cinnamoyltyramine (5),N-trans-coumaroyltyramine (6),N-trans-caffeoyltyramine (7),dihydro-N-caffeoyltyramine (8),N-trans-coumaroyloctopamine (9),N-［2-(3,4-dihydroxyphenyl)-2-hydroxyethyl］-3-(4-methoxyphenyl)prop-2-enamide (10),N-trans-feruloyl-3′-O-methyldopamine (11),dihydro-feruloyl-5-methoxytyramine (12),N-trans-sinapoyltyramine (13),N-acetyl-N′-trans-feruloylputrescine (14),N-acetyltyramine (15).Additionally,compounds 3-5,9-15 were isolated from Lycium yunnanense Kuang for the first time.They were evaluated forthe anti-inflammatory activities.Among them,compounds 4,6,7 and 12 showed significant anti-inflammatory activities with IC₅₀ values of lower than 17.21±0.50 μM.

Key words: Lycium yunnanense, root, amide constituents, anti-inflammatory activities