关键词: 不同产地, 霍山铁皮石斛, 高效液相色谱法（HPLC）, 指纹图谱, 聚类分析

Abstract: In order to study the chemical composition of Huoshan Dendrobium officinale and provide quality identification,quality control of Dendrobium officinale,the contents of total flavonoids,polysaccharides,naringenin and gigantol in Dendrobium officinale were determined by UV spectrophotometry and HPLC.The methanol extracts of Dendrobium officinale from Huoshan and other areas were analyzed by HPLC,similarity calculation was carried out by using "similarity evaluation system of Chinese traditional medicine fingerprint" software and cluster analysis was carried out.The contents of the main chemical components of Dendrobium officinale were varietious in different areas.The contents of total flavonoids,naringenin and gigantol in five batches of Huoshan Dendrobium officinale were higher and the content of polysaccharides in Huoshan Dendrobium officinale was lower.The common pattern of Huoshan Dendrobium officinale was established.The similarity of the five batches of Huoshan Dendrobium officinale was between 92.6% and 94.8%,compared with the control spectrum of Huoshan Dendrobium officinale.The similarity of the Dendrobium officinale samples from Lin'an,Yueqing,Yancheng and Kunming was between 17.5% and 41.5%.The total peak area of five batchesof Huoshan Dendrobium officinale (9 827.40-14 102.28) were higher than Dendrobium officinale in other areas (730.27-4 206.20).The cluster analysis of the total peak area of different HPLC fingerprints showed that the samples can be divided into two categories,Huoshan Dendrobium officinale as a class,others as one class.There are some differences between the Huoshan Dendrobium officinale and Dendrobium officinale from other areas,the contents of small compound of Huoshan Dendrobium officinale were higher.The result could provide reference for further study on the chemical constituents of Huoshan Dendrobium officinale,and provide experimental evidence for the identification,quality control and comprehensive utilization of Dendrobium officinale resources.

Key words: different areas, Huoshan Dendrobium officinale, high performance liquid chromatography (HPLC), fingerprint, cluster analysis