天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (7): 1219-1225.doi: 10.16333/j.1001-6880.2018.7.021

• 开发研究 • 上一篇    下一篇

近红外光谱技术鉴别白及粉及其混伪品

鄢玉芬,李峰庆,刘珈羽,郭换,陈鸿平*,刘友平*   

  1. 成都中医药大学药学院 中药材质量标准化教育部重点实验室 四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地,成都 611137
  • 出版日期:2018-08-08 发布日期:2018-08-08
  • 基金资助:

    四川省科技厅科技支撑项目(2016SZ0038);成都市科技局科技项目(2014-HM01-00407-SF)

Identification Bletillae Rhizoma and Mixed Pseudo-Powder Samples Based on Near-Infrared Spectroscopy

YAN Yu-fen,LI Feng-qing,LIU Jia-yu,GUO Huan,CHEN Hong-ping*,LIU You-ping*   

  1. Pharmacy College,Chengdu University of Traditional Chinese Medicine,Breeding Base of State Key Laboratory of Resources System Research and Development Utilization of Chinese Herbal Medicines Co-constuctioned by Ministry of Science and Technology and Sichuan Province,Chengdu 611137,China
  • Online:2018-08-08 Published:2018-08-08

摘要: 本研究通过近红外光谱仪采集白及不同品种及混伪粉样品的近红外光谱图后,采用聚类分析方法分别建立白及品种及其混伪品的定性鉴别模型,并进行模型的外部验证和内部验证。最终在波段范围为4 400~4 800、5 400~6 600、7 800~10 000 cm-1白及与其同属植物黄花白及、小白及能够分别聚为一类,内部验证准确率达到100%,外部验证准确率达到90%;在波段5 000~10 000 cm-1范围内白及其伪粉也能够分别聚为一类,光谱属性残差都为0,内部验证和外部验证的准确度均为100%。该技术为快速鉴别白及同属药材的鉴别及混伪粉提供了依据。

关键词: 白及粉, 近红外光谱技术, 定性鉴别, 聚类分析

Abstract: In this study,near-infrared spectroscopy was used to collect the near-infrared spectra of different varieties Bletillae Rhizoma and mixed pseudo-powder samples.Then,the qualitative analysis models of Bletillae Rhizoma and mixed pseudo-powder samples were established by cluster analysis.Moreover,external and internal validation of the model were also experimented.Bletilla striata (Thunb.) Reichb.f.,Bletilla ochracea Schltr.,Bletilla formosana (Hayata) Schltr.can be grouped in the wavelength range of 4 400-4 800,5 400-6 600,7 800-10 000 cm-1.The internal and external verification accuracy reached 100% and 90% respectively.In the range of 5 000-10 000 cm-1,mixed pseudo-powder samples and Bletilla striata (Thunb.) Reichb.f.can also be clustered into one group.The residuals of the spectral properties are both 0,and the internal and external verification accuracy are both 100%.This technology provides the basis for identification Bletilla striata (Thunb.) Reichb.F,same genus medicine and mixed pseudo-powder samples.

Key words: Rhizoma Bletillae powder, NIRS, qualitative identification, cluster analysis

中图分类号: 

O657.33