关键词: 附子, 道地性, 水溶性生物碱, 尼奥灵, HPLC-ELSD, 质量控制

Abstract: This research established an HPLC-ELSD method for determination the several water-soluble alkaloids including fuziline,neoline,songorine in 42 batches crude Fuzi from 4 habitats and 9 batches Fupian (including Sheng Fupian,Chao Fupian,Zheng Fupian) and constructed OPLS-DA model using SIMCA11.0 software for expolre the correlation between the content of water-soluble alkaloids and the geo-authentic.The contents of three alkaloids in samples from the four producing areas are significantly different.The content of neoline in samples from JY are the most abundant,with the average content approximately 2.5 times higher than that from Yunnan and Butuo samples.The distribution characteristics of three alkaloids in Sheng Fupian,Chao Fupian,Zheng Fupian processed by Jiangyou Fuzi are similar to those of crude Jiangyou Fuzi,and there is no significant difference in the content of three alkaloids between the Sheng Fupian,Chao Fupian,Zheng Fupian,in which the content of neoline from Sheng Fupian,Chao Fupian have no significant difference with crude Fuzi.OPLS-DA were used to distinguish Jiangyou Fuzi from non-geo-authentic Fuzi.Two major components (including neoline,which were quantitatively determined) were screen to be characteristic components for the discrimination of Jiangyou Fuzi samples from various regions.The above research results indicate that neoline is related to geo-authentic of Fuzi and can be used as a critical component for identifying geo-authentic Fuzi.Moreover,the content of neoline in Fupian is stable and easy to control.So,neoline can be used as an index to evaluation the quality of Fupian.This paper provides reference for the quality control of Fuzi and Fupian.