关键词: 唐古特大黄, HPLC-DAD法, 切片厚度, 烘干温度, 总蒽醌

Abstract: The aim was to carry out a study about the effect of concentrations of anthraquinone derivatives detected in different thickness of the Rheum tanguticum Maxim. ex Balf. by using high-performance liquid chromatography-photodiode array detector (HPLC-DAD).The condition was Column Agilent Eclipse plus C₁₈(4.6×250 mm,5 μm) with the mobile phase consisting of methanol (A) and 0.1% acetic acid (B) in a gradient elution mode.The column temperature was 25 ℃ and the detection wavelength was 254 nm.The results showed that 75 ℃ drying,sliced 0.4-2 cm or 6-7 cm or 50 and 25 ℃ drying,sliced 0.4-3 cm or 6-8 cm,concentrations of anthraquinone derivatives detected in different thickness of the Rheum tanguticum Maxim. ex Balf. were higher,the contents were lower for other thickness,the contents of aloe-emodin,rhein,emodin,emodin,chrysophanol were in line with the above rules.To guarantee the quality of Rheum tanguticum Maxim. ex Balf.,the proposed primary processing of Rheum tanguticum Maxim. ex Balf. was:dried at 75 ℃ drying,sliced 0.4-2 cm or 6-7 cm or 50 and 25 ℃ drying,sliced 0.4-3 cm or 6-8 cm.

Key words: Rheum tanguticum Maxim. ex Balf.；HPLC-DAD, different thickness；drying temperature, anthraquinone derivatives