天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (2): 185-190.doi: 10.16333/j.1001-6880.2019.2.001

• 研究论文 •    下一篇

超声-微波协同提取大青叶多糖及其分子特性和免疫活性研究

曹荣安1,2，李朝阳3，李昌盛4，张开江5，王长远1*   

  1. 1黑龙江八一农垦大学食品学院；2黑龙江省农产品加工与质量安全重点实验室；3黑龙江八一农垦大学国家杂粮工程技术研究中心，大庆 163319；4韩国江陵原州大学海洋食品科学技术系，江陵 210702；5大庆市燃乏中药材种植专业合作社，大庆 163411
  • 出版日期:2019-03-07 发布日期:2019-03-07
  • 基金资助:

    大庆市指导性科技计划（zd-2016-148，zd-2017-54)；黑龙江省教育厅项目（2016-KYYWF-0171）；黑龙江八一农垦大学学成、引进人才科研启动计划（XDB2015-30）；黑龙江八一农垦大学“三横三纵”科研团队支持计划（TDJH201806）

Ultrasound-microwave assisted extraction,molecular properties and immunomodulatory activities of the polysaccharide from Folium isatidis

CAO Rong-an1,2, LI Chao-yang3, LI Chang-sheng4, ZHANG Kai-jiang5, WANG Chang-yuan1*   

  1. 1College of Food Science,Heilongjiang Bayi Agricultural University,Daqing 163319,China; 2Key Laboratory of Agro-Products Processing and Quality Safety of Heilongjiang province,Daqing 163319,China; 3National Coarse Cereals Engineering Research Center,Heilongjiang Bayi Agricultural University,Daqing 163319,China; 4Department of Marine Food Science and Technology,Gangneung-Wonju National University,Gangneung 210702,South Korea; 5Daqing RanFa specialty cooperative of Chinese medical herb planting,Daqing 163411,China
  • Online:2019-03-07 Published:2019-03-07

PDF (PC)

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摘要： 为研究大青叶多糖的分子特性和免疫活性，采用超声-微波协同提取大青叶多糖并测定其化学组成，衍生化后利用气质联用分析仪检测单糖组成和链接方式，用HPSEC-MALLS-RI联机系统测定分子质量及分布情况，最后利用RAW264.7细胞分析大青叶多糖的免疫活性。结果表明，多糖最佳提取参数：料液比25∶1（V/m），温度90 ℃，蒸馏水浸提70 min，超声-微波提取50 min，微波功率500 W，超声功率50 W，多糖得率为10.49%。大青叶多糖化学组成包括63.8%总糖、13.1%蛋白质、14.2%硫酸根和12.6%糖醛酸，单糖组成主要包括半乳糖、阿拉伯糖、鼠李糖和葡萄糖，分子质量为7.85×105 u，回转半径为183.3 nm。同时分析了大青叶多糖的链接方式，体外生物活性实验表明大青叶多糖可以促进RAW264.7细胞增殖并产生一氧化氮。

关键词: 大青叶, 多糖, 超声-微波, 分子特性, 免疫活性

Abstract: The molecular properties and immunomodulatory activities of the polysaccharide were investigated in this study,which was extracted from Folium isatidis by the distilled water with the assistance of ultrasound-microwave.Firstly,the chemical compositions of the polysaccharide were analyzed,and its monosaccharide composition and glycosidic linkage were investigated by GC-MS after derivatization.The molecular weight assay was conducted using HPSEC-MALLS-RI system.Finally,the immunomodulatory activities of the polysaccharide were carried out using RAW264.7 cells.In conclusion,the optimal extraction parameters studied by the orthogonal experiment,were as follows,ratio of liquid to solid 25∶1 (V/m),temperature 90 ℃,distilled water extraction 70 min,ultrasound-microwave extraction time 50 min,microwave power 500 W,ultrasound power 50 W,with the yield of polysaccharide 10.49%.The polysaccharide contained 63.8% neutral sugar,13.1% protein,14.2% sulfate and 12.6% uronic acid.Its major monosaccharide units were galactose,arabinose,rhamnose and glucose.The Mw and Rg of the polysaccharide were 7.85×105 u and 183.3 nm,respectively.The glycosidic linkage of polysaccharide was also investigated.According to the results of immunomodulatory activities,the polysaccharide can enhance the proliferation of RAW264.7 to produce NO.

Key words: Folium isatidis, polysaccharide, ultrasound-microwave, molecular properties, immunomodulatory activities

中图分类号: 

R284.2

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