关键词: 超高效液相色谱-四级杆飞行时间质谱法, 百合珠芽, 薯蓣皂苷元, 抗肿瘤

Abstract: To establish a method for rapid identification of 10 components in bulbil of Lilium lancifolium Thunb.by UPLC-Q-TOF-MS,and carry out research of tumor cell proliferation study of diosgenin.A Thermo Hypersil Gold C₁₈ column (100 mm×2.1 mm,1.9 μm) was used with the mobile phase of acetonitrile (A)-0.5% acetic acid (B) by gradient elution.Positive and negative ions were simultaneously monitored by electro-spray ionization (ESI),and CCK-8 assay was used to screen the effect of diosgenin on lung cancer A549 cells and gastric cancer HGC-27 cells.Based on the fragmentation pattern and reference results,the structures of 10 compounds including p-coumaric acid,diosgenin and quercetin were identified.The pharmacological study showed that diosgenin had only a weak inhibitory activity on the proliferation of A549 cells in lung cancer,and had a strong inhibitory effect on the proliferation of HGC-27 cells in gastric cancer.These results suggested that UPLC-Q-TOF-MS/MS can be used in the qualitative analysis of the main components of bulbil of Lilium lancifolium Thunb.from retention time,UV absorption spectrum,precise relative molecular weight,molecular formula and secondary fragment ions,and so on.Meanwhile,diosgenin have a strong inhibitory effect on the proliferation of gastric cancer HGC-27 cells.This research provided a scientific basis for the ues of bulbil of Lilium lancifolium Thunb.．

Key words: UPLC-Q-TOF-MS, bulbil of Lilium lancifolium Thunb., diosgenin, anti-tumor