关键词: 双参, 多糖, 单糖组成, 抗氧化活性

Abstract: To study the effect of different alcohol precipitation on the composition and antioxidant activity of Triplostegia glandulifera polysaccharides.Water-soluble components was obtained by boiling water extraction,deproteinized,and macroporous resin column chromatography,then the crude polysaccharides of TGP-1,TGP-2 and TGP-3 were obtained by 40%,60% and 80% alcohol precipitation.PMP-HPLC methodology was used to analyze the monosaccharide compositions of TGP-(1-3).Finally,their antioxidant capacity was studied by scavenging of DPPH,hydroxyl and superoxide anion free radical method.The results showed that the polysaccharides TGP-1 and TGP-2 were composed of D-mannose, D-glucosamine hydrochloride,D-galacturonic acid,D-glucose,D-galactose and D-arabinose,the molar ratio of TGP-1 is about 0.06∶0.03∶0.33∶3.90∶0.63∶041,and the TGP-2 is about 0.08∶0.06∶1.05∶15.71∶0.92∶0.49. Moreover,TGP-3 were composed of D-galacturonic acid,D-glucose,D-galactose and D-arabinose and their molar ratio was 0.49∶5.65∶0.50∶0.35. Antioxidant capacity test results showed that the antioxidant capabilities of TGP-(1-3) were obviously different,among which TGP-3 had the strongest antioxidant capacity,and TGP-1 had the weakest antioxidant capacity.

Key words: Triplostegia glandulifera, polysaccharides, monosaccharide composition, antioxidant activity