天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (1): 7-15.doi: 10.16333/j.1001-6880.2022.1.002

• 研究论文 • 上一篇    下一篇

橄榄果渣乙醇提取物成分分析及抗氧化和抑菌性能研究

李春爱,田立鹏,蔡梦,蒲陆梅*   

  1. 甘肃农业大学理学院,兰州 730070
  • 出版日期:2022-01-28 发布日期:2022-01-28
  • 基金资助:
    国家自然科学基金(31860472)

Study on the component,antioxidant and antibacterial properties of ethanol extracts from olive pomace 

LI Chun-ai,TIAN Li-peng,CAI Meng,PU Lu-mei*   

  1. College of Science,Gansu Agricultural University,Lanzhou 730070,China
  • Online:2022-01-28 Published:2022-01-28

摘要: 橄榄果渣中生物活性成分丰富。本文主要研究橄榄果渣提取物成分及其对灰葡萄孢的抑菌性能。首先以橄榄果渣为原料,采用加热回流法,分别以H2O、50%乙醇、70%乙醇和90%乙醇为提取剂,得到四种提取物并采用HPLC和GC-MS对提取物进行成分分析。其次测定了提取物的总酚含量、抗氧化性和对灰葡萄孢的抑菌性能。研究表明,90%乙醇提取物的酚类含量较高,抗氧化水平优良,并且对灰葡萄孢有明显的抑制作用。该研究为橄榄果渣活性成分在抑菌性能方面的研究提供了一定的理论依据。

关键词: 橄榄果渣, 提取物, 抗氧化, 灰葡萄孢, 抑菌性

Abstract:

Bioactive component are rich in olive pomace.This paper mainly researched the component and antibacterial activity against Botrytis cinerea of olive pomace extracts.Firstly,olive pomace were extracted by heating reflux method with H2O,50% ethanol,70% ethanol and 90% ethanol as extractants respectively and four extracts were obtained.And the component of extracts were analyzed by GC-MS and HPLC.Secondly,the extracts of the total phenol and antioxidant activity,antibacterial activity against B. cinerea were investigated.The results showed that among the four extracts,90% ethanol extract had high phenolic content,excellent antioxidant properties and obvious inhibitory effects on B. cinerea.This study provides a theoretical basis for the study of antibacterial properties of active components from olive pomace.

Key words: olive pomace, extracts, antioxidation, Botrytis cinerea, fungistasis

中图分类号:  S476