天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (8): 1314-1321.doi: 10.16333/j.1001-6880.2023.8.004

• 研究论文 • 上一篇    下一篇

山楂叶总黄酮对链脲佐菌素诱导的糖尿病小鼠体内抗氧化酶活性及相关基因表达的影响

陈薇伊,徐兴军*,刘佳人,张伟伟,杨   烨,邵淑丽   

  1. 齐齐哈尔大学生命科学与农林学院 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室 寒区麻及制品教育部工程中心,齐齐哈尔 161006
  • 出版日期:2023-08-28 发布日期:2023-08-30
  • 基金资助:
    黑龙江省省属本科高校基本科研业务费面上项目(135509133)

Effect of total flavonoids from hawthorn leaf on antioxidant enzyme activity and related gene expression in streptozotocin-induced diabetic mice

CHEN Wei-yi,XU Xing-jun*,LIU Jia-ren,ZHANG Wei-wei,YANG Ye,SHAO Shu-li    

  1. Department of Life Science and Agroforestry,Qiqihar University; Key Laboratory of Resistance Genetic Engineering and Biodiversity Conservation in Cold Regions of Heilongjiang Province; Engineering Center of Ministry of Education of Linen and Linen Products in Cold Regions,Qiqihar 161006,China
  • Online:2023-08-28 Published:2023-08-30

摘要:

糖尿病严重危害人类健康,其发病机制与氧化应激有关。山楂(Crataegus pinnatifida Bunge)作为药食兼用植物,其中的山楂叶总黄酮(total flavonoids from hawthorn leaf,TFHL)具有良好的抗氧化性。为了研究山楂叶总黄酮对糖尿病小鼠的抗氧化作用,以84只4周龄SPF级雄性ICR小鼠作为实验研究对象,将其随机分为空白对照组、模型对照组、DMSO模型对照组、山楂叶总黄酮高、中、低浓度组、阳性对照组,组间体质量差异不显著。各模型组小鼠以150 mg/kg一次性腹腔注射1%的链脲佐菌素(streptozotocin,STZ),构建STZ诱导的糖尿病小鼠模型,空白对照组注射等量的柠檬酸钠溶液。模型制备成功后,每天定时灌胃,灌胃28 d。试剂盒法检测小鼠血清和肝脏组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、总抗氧化能力(T-AOC)酶活性及丙二醛(MDA)含量,荧光定量PCR法测定抗氧化基因SOD-1、SOD-2、GPX-1、GPX-4表达量。结果表明:山楂叶黄酮能极显著提高糖尿病小鼠血清和肝脏中的SOD、T-AOC、GSH-PX酶活性(P<0.01),极显著降低MDA含量(P<0.01);并显著上调了糖尿病小鼠肝脏中SOD-1、SOD-2、GPX-1和GPX-4基因的表达。山楂叶黄酮能改善STZ诱导的糖尿病小鼠的氧化应激,提高其抗氧化能力。

关键词: 山楂叶黄酮, 糖尿病, 抗氧化, 基因表达

Abstract:

Diabetes is a serious hazard to human health,and its pathogenesis is related to oxidative stress.Hawthorn (Crataegus pinnatifida Bunge) is a fruit-bearing shrub as a medicinal substance,in which total flavonoids from hawthorn leaf (TFHL) have favorable antioxidant properties.To explore the antioxidant effect of TFHL on diabetic mice,84 four-week-old SPF male ICR mice were randomly divided into control group,model control group,DMSO model control group,model groups with high-,medium-and low-dose of TFHL and positive control group,there was no significant difference in body mass among all groups.The mice in each model group were intraperitoneally injected with 1% streptozotocin (STZ) at 150 mg/kg to construct STZ-induced diabetic mice,and the control group were injected with the same amount of sodium citrate solution.After the successful preparation of the model,the patients were given different concentrations of TFHL,or metformin hydrochloride or DMSO or water daily for 28 days.Then,enzyme activities of speroxide dismutase (SOD),glutathione peroxidase (GSH-PX),total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content in serum and liver tissues of mice were measured by the reagent kit method;the expression of antioxidant genes SOD-1,SOD-2,GPX-1 and GPX-4 were detected by Real-time PCR.The results showed that TFHL significantly increased the activities of SOD,T-AOC and GSH-PX in the serum and liver of diabetic mice (P<0.01),and reduced the content of MDA (P<0.01).meanwhile the expression of SOD-1,SOD-2,GPX-1 and GPX-4 genes were increased in the liver of diabetic mice after treated with TFHL.These data suggested that TFHL can improve the oxidative stress in STZ-induced diabetic mice and enhance their antioxidant capacity.

Key words: total flavonoids from hawthorn leaf, diabetes mellitus, antioxidation, gene expression

中图分类号:  R285 R965.1