天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (9): 1495-1504.doi: 10.16333/j.1001-6880.2023.9.004

• 研究论文 • 上一篇    下一篇

基于代谢组学技术分析枸杞愈伤组织抗氧化活性成分差异

曾晓倩，周笑如，刘春环，刘   学*，杨   成   

  1. 江南大学化学与材料工程学院 合成与生物胶体教育部重点实验室，无锡 214122
  • 出版日期:2023-09-22 发布日期:2023-09-21
  • 基金资助:
    江苏省自然科学基金青年项目（BK20221069）

Metabolomics-based analysis on the differences in antioxidant components from callus of Lycium barbarum L.

ZENG Xiao-qian,ZHOU Xiao-ru,LIU Chun-huan,LIU Xue*,YANG Cheng   

  1. Key Laboratory of Synthetic and Biological Colloids,Ministry of Education,School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,China
  • Online:2023-09-22 Published:2023-09-21

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摘要：

采用非靶向代谢组学技术，探究不同植物生长调节剂组合对枸杞愈伤组织抗氧化活性成分富集的影响。以枸杞无菌苗茎段为外植体，分别以0.25 mg/L 1-萘乙酸＋0.125 mg/L 6-苄氨基嘌呤（NB）和0.25 mg/L 2,4-二氯苯氧乙酸＋0.125 mg/L 激动素（DK）为植物生长调节剂进行愈伤组织培养，以DPPH自由基清除法检测不同愈伤组织的抗氧化活性，在相同浓度下NB抗氧化活性是DK的1.9倍。基于UHPLC-Q-TOF-MS的代谢组学技术对两组样品进行代谢物分析，共鉴定出752种代谢物，其中55种的相对含量存在显著差异，主要为氨基酸类和糖类；KEGG分析发现差异代谢通路主要为ABC转运蛋白通路。ABC转运蛋白的功能差异可能是导致枸杞愈伤组织抗氧化活性差异显著的主要原因。

关键词: 枸杞, 愈伤组织, 抗氧化, 代谢组学, 差异代谢物

Abstract:

The effects of different plant growth regulator combinations on the enrichment of antioxidant components in Lycium barbarum L. callus were investigated by non-targeted metabolomics technique.The sterile stem segments of L. barbarum was used as explant,and 0.25 mg/L naphthylacetic acid+ 0.125 mg/L 6-benzylaminopurine (NB) or 0.25 mg/L 2,4-dichlorophenoxyacetic acid+ 0.125 mg/L kinetin (DK) were used as plant growth regulators for callus culture.The antioxidant activity of different callus was detected by DPPH free radical scavenging method.The antioxidant activity of NB was 1.9 times that of DK at the same concentration.Based on the metabolomics technology of UHPLC-Q-TOF-MS,a total of 752 metabolites were identified in two groups of samples,of which 55 had significant differences in relative content,mainly amino acids and carbohydrates;KEGG analysis revealed that the differential metabolic pathway was mainly the ABC transporter pathway.The functional difference of ABC transporters may be the main reason for the significant difference in antioxidant activity of L. barbarum callus.

Key words: Lycium barbarum L., callus, antioxidant, metabolomics, differential metabolites

中图分类号:  R282.6 R914.4

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