This paper presents a comparative study of flavonoid content,in vitro antioxidant activity and lipid-lowering activity of different solvent extracts of Paeonia lactiflora flower. In this paper, the flavonoid content was determined by NaNO₂-Al(NO₃)₃ colorimetric method,the antioxidant capacity of P. lactiflora flower extract was evaluated by DPPH radical scavenging rate,ABTS radical scavenging rate,and total reducing capacity,and the lipid-lowering activity of P. lactiflora flower extract was evaluated by pancreatic lipase inhibition capacity,bile-acid-binding capacity,and cholesterol-adsorption capacity.The results showed that the total flavonoid content of the low-eutectic solvent extract was higher than that of the ethanol extract,and also the low-eutectic solvent extraction method improved the antioxidant activity and lipid-lowering activity of P. lactiflora flower extracts,and the IC₅₀ values of the low-eutectic solvent extracts for the scavenging of ABTS free radicals were 0.256 mg/mL,and for the scavenging of DPPH free radicals,the IC₅₀ values of the low-eutectic solvent extracts for the scavenging of DPPH free radicals were 0.296 mg/mL;and the IC₅₀ values for the inhibition of pancreatic Lipase half inhibitory concentration was 0.788 mg/mL,the IC₅₀ values for sodium cholate,sodium taurocholate and sodium glycinate binding were 0.883,0.841,0.850 mg/mL,respectively,and the maximum adsorption of cholesterol was 6.28 mg/g when the dosage of P. lactiflora flower crude extract was 0.5 g. Therefore,low eutectic solvent can be used as a suitable extraction solvent for extracting antioxidant and lipid-lowering components from P. lactiflora flower,and the extracts have better antioxidant and lipid-lowering potentials,which can provide theoretical basis for the subsequent high-value utilization of P. lactiflora flower.