This study aims to study the chemical constituents of Cinnamomum camphora chvar.Borneol branches.Three compounds were isolated and purified from the ethyl acetate extraction of C. camphora chvar.Borneol branches by silica gel,Sephadex LH-20,MCI column chromatography and preparative high-performance liquid chromatography.Their chemical structures were identified as campholactone A (1),northalifoline (2),N-phenylethylbenzamide (3) by spectral techniques and camparison with literature data.Compound 1 is a new eucalyptus sesquiterpene lactone with a rare amide substitution at the C-11 position.Compounds 2 and 3 were isolated for the first time from titled plant.The anti-inflammatory activities of compounds 1-3 were evaluated by NO release in LPS-induced RAW 264.7 macrophages and Griess assay,the antitumor activities of compounds 1-3 against human liver cancer HepG2 cells were performed by the MTT method.The experimental results showed that compounds 1-3 exhibited varying degrees of inhibitory effects on HepG2 at a concentration of 20 μmol/L,with inhibition rates of 35.78%±0.15%,42.36%±0.58% and 23.95%±0.40%,respectively;compounds 1-3 weakly inhibited the secretion of nitric oxide (NO) with an inhibition rate of less than 20%.

Cinnamomum camphora chvar.Borneol, eudesmane-type sesquiterpenoid lactone;chemical constituents;structure identification