To enhance the extracting amount of flavonoids in Scutellariae Indicae Herba,an experiment employed deep eutectic solvents(DESs) choline chloride-ethylene glycol approach to extract flavonoids from Scutellariae Indicae Herba.The extracting process was optimized through a combination of single factor experiment and Plackett-Burman design along with Box-Behnken response surface methodology,and the influential factors such as moisture content,liquid-solid ratio,and ultrasound power were utilized to assess the extraction yield of total flavonoids in Scutellariae Indicae Herba.The results demonstrated that the optimal extracting conditions were a moisture content of 59.30%,a liquid-solid ratio of 25.90∶1,and an ultrasound power of 121 W,with amount of flavonoids 7.924 mg/g.Under the same circumstances,the extracting efficiency of flavonoids using choline chloride-ethylene glycol as the extraction solvent was significantly higher than that of other conventional solvents,and the advantage of efficient extracting amount of flavonoids was attained.Hence,the optimized ultrasound-assisted deep eutectic solvents choline chloride-ethylene glycol method can conspicuously improve the extracting amount of flavonoids.