The outbreak and prevalence of Vibrio alginolyticus caused huge economic losses on marine aquaculture,with the formation of biofilms exacerbating the pathogenicity and antibiotic resistance.At present,Chinese herbal medicines has shown positive potential in the prevention and treatment of pathogenic bacterial infections.To further explore this potential,a study was conducted which 19 Chinese herbal medicines (Lycii Fructus,Paridis Rhizoma,Asteris Radix et Rhizoma,Polygoni Mul Tiflori Radix,Spatholobi Caulis,Magnoliae Officinalis Cortex,Ecliptae Herba,Desmodii StyracifolII Herba,Gardeniae Fructus,Downy Rosemyrtle,Forsythiae Fructus,Caryophylli Flos,Solani Lyrati Herba,Phellodendri Chinensis Cortex,Artemisiae Argyi Folium,Anemarrhenae Rhizoma,salt processed Anemarrhenae Rhizoma,wine processed Anemarrhenae Rhizoma,stir-frying processed Anemarrhenae Rhizoma) were carefully selected and subjected to extraction using various solvents to explore the effects of these extracts on the growth and biofilm formation of V. alginolyticus. The effects of these extracts on the growth of V. alginolyticus were systematically assessed through the utilization of the Oxford cup method and the 96-well plate method.Additionally,the potential influence of sub-inhibitory concentrations of these herbal extracts on biofilm formation of V. alginolyticus was investigated by means of crystal violet staining.The findings indicated that water extracts of 12 Chinese herbal medicines,alcoholic extracts of 17 Chinese herbal medicines and ester extracts of 15 Chinese herbal medicines displayed noteworthy antibacterial activity.Significantly,the water extracts of Caryophylli Flos,alcoholic extracts of Paridis Rhizoma and Anemarrhenae Rhizoma,and ester extracts of Downy Rosemyrtle and Forsythiae Fructus demonstrated noteworthy inhibitory effects on the formation of biofilm by V. alginolyticus.Furthermore,distinctions in antibacterial activity were discerned between the unprocessed and processed forms of Anemarrhenae Rhizoma,despite their some similar impacts on biofilm formation.The results of this investigation carry substantial guiding significance for the prevention and management of bacterial infections and the development of agents that counteract biofilm formation.These findings are expected to provide new and sustainable strategies for responding to outbreaks of V. alginolyticus,helping to protect the sustainability of marine aquaculture,safeguard food safety,and safeguard human health.