The content determination method and fingerprint evaluation method of Polygoni Multiflori Caulis were established.The quantity value transfer rule from Polygoni Multiflori Caulis (PMC) decoction pieces to PMC the standard decoction of Chinese materia medica was studied.Twenty batches of PMC from the main producing areas and authentic producing areas in China were collected.Prepared into decoction pieces of PMC in accordance with the provisions of the 2020 edition of Chinese Pharmacopoeia,and the standard decoction of Shouwuteng was prepared according to "Technical Requirements for Quality Control and Standard Formulation of Chinese Medicine Formula Granules".HPLC was used to establish the content determination method and fingerprint method.The transfer rate,extraction rate and similarity of fingerprint were taken as indexes,the quantity value transfer rule from PMC decoction pieces to PMC standard decoction was analyzed.Results showed that the established HPLC method and fingerprint method were suitable for the determination of PMC decoction pieces and its standard decoction.The results showed that the paste rate of 20 batches of PMC standard decoction was 10.4%-16.1%,the mean value was 12.9%,all were within ± 30% of the mean value.In addition to the samples of Sichuan origin,The HPLC fingerprint similarity of other origin of PMC decoction pieces and PMC standard decoction of Chinese materia medica were all good (> 0.9),And they have 6 peaks in common,Four of them were identified as 2,3,5,4-tetrahydroxystilbene-2-O-β-D-glucoside,emodin-8-O-β-D-glucoside,emodin,emodin-3-methyl ether.The transfer rates of the four known components were 26.58%-53.07% (2,3,5,4-tetrahydroxystilbene-2-O-β-D-glucoside),12.37%-42.84% (emodin-8-O-β-D-glucoside),3.83%-18.71% (emodin) and 0.35%-4.13% (emodin-3-methyl ether).Therefore,a method for the content and fingerprint of PMC and its standard decoction have been established,and the quantity value transfer law of chemical composition between them was expounded.The reference was provided for the quality control of water extract and formula granule of PMC.