天然产物研究与开发 ›› 2024, Vol. 36 ›› Issue (7): 1175-1181.doi: 10.16333/j.1001-6880.2024.7.009

• 研究简报 • 上一篇    下一篇

桂林会仙湿地真菌Talaromyces sp. W21的化学成分及其抗炎活性研究

梁   琳1，袁   玺2，冼小雅1，周先丽2，王力生1，邝彤东3，梁成钦1*   

  1. 1桂林医学院药学院；2桂林医学院智能医学与生物技术学院；3桂林医学院 广西糖尿病系统医学重点实验室，桂林 541199
  • 出版日期:2024-07-28 发布日期:2024-07-26
  • 基金资助:
    国家自然科学基金（81860621）

Chemical constituents and anti-inflammatory activity of Talaromyces sp. W21 from Huixian Wetland in Guilin

LIANG Lin1,YUAN Xi2,XIAN Xiao-ya1,ZHOU Xian-li2,WANG Li-sheng1,KUANG Tong-dong3,LIANG Cheng-qin1*   

  1. 1School of Pharmacy,Guilin Medical University; 2School of Intelligent Medicine & Biotechnology,Guilin Medical University; 3Guangxi Key Laboratory of Diabetic Systems Medicine,Guilin Medical University,Guilin 541199,China
  • Online:2024-07-28 Published:2024-07-26

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摘要：

研究桂林会仙湿地来源真菌Talaromyces sp.W21的化学成分及其抗炎活性。综合运用多种柱色谱、现代波谱技术和理化性质对Talaromyces sp.W21发酵提取物中的化学成分进行分离、纯化和结构鉴定，并从中分离得到10个化合物，分别鉴定为cytospone K（1）、(3S,4R,5R)-4-hydroxymethyl-3,5-dimethyldihydro-2-furanone（2）、lasdiplactone（3）、leptosphaerone C（4）、annularin A（5）、annularin K（6）、cladobotrin V（7）、2-(2′S-hydroxypropyl)-5-methyl-7-hydroxychromone（8）、bacillisporin A（9）、bacillisporin B（10）。其中，化合物1为新的α-吡喃酮类化合物，化合物2~8为首次从Talaromyces sp.中分离获得。抗炎活性实验结果表明化合物9和10对LPS诱导的RAW 264.7释放NO具有一定的抑制作用，其IC50分别为38.90 ± 0.79、35.02 ± 1.03 μmol/L。

关键词: 湿地真菌, 化学成分, α-吡喃酮, 抗炎活性

Abstract:

This study aims to investigate the chemical constituents and anti-inflammatory activity of Talaromyces sp. W21 from the Huixian wetland in Guilin.A new α-pyrone derivative,namely cytospone K (1),together with nine known compounds,namely (3S,4R,5R)-4-hydroxymethyl-3,5-dimethyldihydro-2-furanone (2),lasdiplactone (3),leptosphaerone C (4),annularin A (5),annularin K (6),cladobotrin V (7),2- (2′S-hydroxypropyl) -5-methyl-7-hydroxychromone (8),bacillisporin A (9) and bacillisporin B (10) were isolated and purified from cultures of Talaromyces sp. W21 by using various column chromatography.Their structures were identified by modern spectroscopic techniques and physicochemical properties.Compound 1 was a new α-pyranone derivative,and compounds 2-8 were isolated from the Talaromyces sp. for the first time.The anti-inflammatory activity assay showed that compounds 9 and 10 have a moderate inhibition of NO production induced by LPS in RAW 264.7 cells,with IC50 values of 38.90 ± 0.79 and 35.02 ± 1.03 μmol/L respectively.

Key words: wetland fungus, chemical constituents, α-pyrone derivative, anti-inflammatory activity

中图分类号:  R284.1

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