天然产物研究与开发 ›› 2010, Vol. 23 ›› Issue (6): 1048-1052.

• 研究简报 • 上一篇    下一篇

云南松健康松与衰弱松针叶挥发物化学成分比较

伍苏然1,周平阳2,李正跃1,付瑞金1,袁盛勇3,肖 春1*   

  1. 1云南农业大学植保学院,昆明650201;2云南省德宏州森防站,潞西 678400; 3红河学院农学系,蒙自661100
  • 收稿日期:2009-02-13 出版日期:2011-01-10 发布日期:2011-10-19
  • 通讯作者: x.chun@163.com
  • 基金资助:

    云南省科技厅重点攻关项目(2005NG03)

Analysis the Volatile Constituents of Pine Needles from Health and Debility Pinus yunnanensis

WU Su-ran1,ZHOU Ping-yang2,LI Zheng-yue1,FU Rui-jin1,YUAN Sheng-yong3,XIAO Chun1   

  1. 1Faculty of Plant Protection, Yunnan Agricultural University, Kunming 650201,China; 2Dehong station of Forestry Plant, Luxi 678400, China; 3Department of Agronomy, Honghe University, Mengzi 661100,China

  • Received:2009-02-13 Online:2011-01-10 Published:2011-10-19

摘要:

采用顶空吸附法对健康松、衰弱松的针叶进行了活体挥发性物质收集。通过GC-MS联用技术和计算机信息检索法对两种状态下松树的针叶挥发物化学成分进行了分离鉴定,用离子流峰面积归一法通过化学工作站数据处理系统确定了各成分的相对含量。比较结果表明:云南松由健康松转向衰弱松的过程中挥发性成分及含量发生了很大变化。两种状态下针叶挥发性物质中共有15 种相异化合物成分,18 种相同化合物成分,相同化合物成分在含量上存在很大差异。

关键词: 云南松, 针叶, 挥发物, 化学成分, 气相色谱-质谱联用仪

Abstract:

The volatile oils of healthy and debility pine needles of Pinus yunnanensis were collected using head-space adsorption method. Chemical constituents of oils in two states were isolated and identified by using gas chromatography-mass spectrometry GC-MS and computer information searching. The relative contents of the volatile constituents were calculated by ionic flow chromatographic area normalization method. The results showed that the constituents and contents of volatiles changed largely during the process from healthy to debility. There were 15 different constituents and 18 same constituents between two states pine needles. The relative contents of the same constituents of two states pine needles were different.

Key words: Pinus yunnanensis, pine needles, volatiles, chemical constituents, GC-MS