从盾叶薯蓣组培苗中高压酸解制备薯蓣皂苷元

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (1): 114-117.

从盾叶薯蓣组培苗中高压酸解制备薯蓣皂苷元

阴春晖，李培琴，赵江林，单体江，何桂玲，周立刚^*

中国农业大学农学与生物技术学院，北京100193

收稿日期:2010-07-05 发布日期:2011-07-20
通讯作者: lgzhou@cau.edu.cn
基金资助:
北京市自然科学基金（6092015），国家自然科学基金（30871662，31071710）

Acidolysis Preparation of Diosgenin at High Pressure from the Cultured Plantlets of Dioscorea zingiberensis

YIN Chun-hui, LI Pei-qin, ZHAO Jiang-lin, SHAN Ti-jiang, HE Gui-ling, ZHOU Li-gang^*

College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China

Received:2010-07-05 Published:2011-07-20

摘要/Abstract

摘要：

采用正交试验法对盾叶薯蓣（Dioscorea zingiberensis）组培苗中薯蓣皂苷元的高压酸解制备工艺进行了研究。以薯蓣皂苷元的含量作为评价指标，选用正交表L16(45)，以样品用量、硫酸浓度、提取时间为因素，设计了3因素4水平的正交试验。结果表明：高压酸解提取薯蓣皂苷元的最佳工艺条件为：样品用量25 mg、硫酸浓度0.5 mol/L、提取时间2 h，在此条件下提取物中薯蓣皂苷元的平均含量为9.12 mg/g。

关键词: 盾叶薯蓣, 薯蓣皂苷元, 高压酸解, 正交试验

Abstract:

Acidolysis preparation at high pressure of diosgenin from the cultured plantlets of Dioscorea zingiberensis was studied by L₁₆(4⁵) orthogonal experiments. The effects of sample quantity, concentration of sulfuric acid, and extraction time were investigated. The results showed that diosgenin content of the plantlets reached 9.12 mg/g under the optimum extraction condition that was 25 mg of sample, 0.5 mol/L of sulfuric acid, and 2 h of extraction time.

Key words: Dioscorea zingiberensis, diosgenin, acidolysis preparation at high pressure, orthogonal experiments

阴春晖，李培琴，赵江林，单体江，何桂玲，周立刚. 从盾叶薯蓣组培苗中高压酸解制备薯蓣皂苷元[J]. 天然产物研究与开发, 2011, 23(1): 114-117.

YIN Chun-Hui, LI Pei-Qin, ZHAO Jiang-Lin, DAN Ti-Jiang, HE Gui-Ling, ZHOU Li-Gang. Acidolysis Preparation of Diosgenin at High Pressure from the Cultured Plantlets of Dioscorea zingiberensis[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(1): 114-117.

参考文献

1 Huang YH(黄亚辉),Sheng XB(盛孝邦).Progress and prospect on botanical research of Dioscorea zingiberensis.Chin J Chin Mater Med (中国中药杂志),2005,30:1805-1808.
2 Ding ZZ(丁志遵),Tang SR(唐世蓉),Qin HZ(秦慧贞).Medicinal Plants for Producing Steroid Hormones (甾体激素药源植物).Beijing:Science Press,1983.95.
3 Li JC(李军超),Li XM(李向民),Guo XS(郭晓思),et al.Advances in study of Dioscorea zingiberensis.Acta Bot Borea1-Occident Sin (西北植物学报),2003,23:1842-1848.
4 Wang FZ(王凤芝),Liu J(刘娟).Different extract methods of diosgenin.Helongjiang Med Pharm (黑龙江医药科学),2006, 29: 22-23.
5 Hua HJ(华怀杰),Zhong SA(钟世安),Wang YM(王炎伟),et al.A new technology for extracting diosgenin from Dioscorea zingibersis.Nat Prod Res Dev (天然产物研究与开发),2007,19:306-308.
6 Zhu X(朱宪),Wang ZW(王振武),Guo XY(郭晓亚).Technology of diosgenin production by dioscin hydrolysis in subcritical water.J Chem Eng Chin Univ (高校化学工程学报),2007,21:122-125.
7 Han F(韩枫),Li WH(李稳宏),Li D(李冬),et al.Extraction of diosgenin from Dioscorea zingiberensis.Chin Tradit Patent Med (中成药),2007,29:1765-1768.
8 Zhang WG(张惟广),Ren Y(任雁).Study on the extraction of Chinese yam with direct fermentation. Sichuan Food Ferment (四川食品与发酵),2008,44:31-35.
9 Wang YL(王元兰),Li SF(李水芳),Yang Z (杨志).A new technology for extracting diosgenin from Dioscorea zingiberensis. Economic Forest Res,2002,20:67-68.
10 Wang J(王俊),Chen J(陈钧),Yang KD(杨克迪),et al.Research on etraction of diosgenin by original spot hydrolyzedninhydrin. Chin J Chin Mater Med (中国中药杂志),2003,28:934-936.
11 Zhang LM(张黎明),Zhang LY(张露亿),Du LX(杜连祥).Technology for extracting diosgenin from seeds of Trigonella foenum-graecum through enzymatic hydrolysis.T CSAE (农业工程学报),2005,21:161-164.
12 Zhang LM(张黎明),Xu W(徐玮).Research on ultrasonic cracking glycoside bond of dioscin from Dioscorea nipponica Makino.Nat Prod Res Dev (天然产物研究与开发),2007,19:286-289.
13 Jiang ZQ(蒋中权),Wang YH(王元华),Zhou C(周超).Hydrolysis technology for diosgenin preparation from Dioscorea zingiberensis (一种利用黄姜加工皂素的水解工艺).CN200510018646.3,2005-4-27.
14 Zhu Y,Ni J,Huang W.Process optimization for the production of diosgenin with Trichoderma reesei.Bioprocess Biosyst Eng, 2010,33:647-655.
15 Xuan SJ(袁绍杰),Liang YL(梁艳丽),Zhao QS (赵庆生),et al.The research progress of the Dioscorea zingiberensis Wright. Southwest China J Agr Sci (西南农业学报),2004,17(S):355-358.
16 Zhou L,Wu J.Development and application of medicinal plant tissue cultures for production of drugs and herbal medicinals in China.Nat Prod Rep,2006,23:789-810.
17 Zhang R(张瑞芬),Li P(李培琴),Zhao J(赵江林),et al.Endophytic fungi from Dioscorea zingiberensis and their effects on the growth and diosgenin production of the host plant cultures.Nat Prod Res Dev (天然产物研究与开发),2010,22:11-15.

[1]	李超, 刘云华, 秦登云, 黄志芳, 刘玉红, 陈燕, 易进海. 基于正交设计优化新鲜川芎中挥发油提取工艺[J]. 天然产物研究与开发, 2024, 36(5): 771-778.
[2]	王庆婷, 耿晓桐, 李雅静, 张娟, 刘庆普, 龚海燕, 雷敬卫, 谢彩侠. 盾叶薯蓣响应低磷胁迫的生理代谢及基因表达特征分析[J]. 天然产物研究与开发, 2023, 35(9): 1569-1582.
[3]	温馨, 胡锦航, 程敏, 宋忠兴. 基于网络药理学、分子对接技术及体外实验验证探讨龙葵治疗乳腺癌的作用机制[J]. 天然产物研究与开发, 2023, 35(10): 1782-1793.
[4]	陶晓赛1，陈志红1,2，谢彩侠1,2，张娟1,2，龚海燕1,2，刘庆普1,2，雷敬卫1,2. 基于HPLC和NIRS建立快速检测盾叶薯蓣中3种皂苷含量的方法[J]. 天然产物研究与开发, 2020, 32(2): 305-316.
[5]	吴姣*，陈迪安，张璐，孙广庭，边禹. 正交实验法优化当归中三种有效成分的提取工艺[J]. 天然产物研究与开发, 2019, 31(增刊1): 91-96.
[6]	宋志姣，汤丹，李悦，章金龙，罗旺全，项辉，马前涛*. 小白及多糖提取、脱蛋白工艺及抗氧化性研究[J]. 天然产物研究与开发, 2019, 31(8): 1317-1325.
[7]	赵娜，张红星*，谢远红，金君华，贾宇. 一株高产Monacolin K紫红曲霉菌株筛选、鉴定及发酵条件优化[J]. 天然产物研究与开发, 2019, 31(8): 1326-1331.
[8]	袁志鹰1,2，罗林明1,陈乃宏1,2,3，梁晟4，周小江1,2，黄惠勇1，陈妍羽1. 基于UPLC-Q-TOF-MS法分析百合珠芽化学成分及其薯蓣皂苷元抗肿瘤活性研究[J]. 天然产物研究与开发, 2019, 31(5): 809-814.
[9]	王蔚，黄旭建，易国春，许思敏，郭雅玲*. HS-GC/MS法分析乌龙茶挥发性成分[J]. 天然产物研究与开发, 2019, 31(2): 222-229.
[10]	刘元林1, 田晓静1, 龙鸣1, 陈士恩1, 李明生1, 2, 高丹丹1, 马忠仁1, 2. 微波辅助提取枸杞多糖工艺优化及内部品质综合判别[J]. 天然产物研究与开发, 2018, 30(增刊2): 194-199.
[11]	胡彦周, 丁轲, 韩涛, 陈湘宁. 基于液质联用分析方法对酸枣仁中欧鼠李叶碱（Frangufoline）提取方法的研究[J]. 天然产物研究与开发, 2018, 30(增刊1): 45-50.
[12]	王宣东, 李策, 康梅松, 田淑雨, 鹿士峰, 吴杨洋, 杜秀菊. 金耳多糖纤维素酶提工艺优化及其初步结构研究[J]. 天然产物研究与开发, 2018, 30(增刊1): 57-64.
[13]	王睿1，赵春草1，吴飞1，徐燕丰2，车京梅2，张继全1*. 正交试验优选芪榔方的水提醇沉工艺[J]. 天然产物研究与开发, 2017, 29(增刊2): 361-366.
[14]	曾聪彦*，张瑜，卓斌，梁食. 正交设计法对脑脉醒神胶囊喷雾干燥工艺的研究[J]. 天然产物研究与开发, 2017, 29(增刊2): 367-369.
[15]	石江林，荣永海，荣龙* . 玫瑰花色苷和花色苷元分离提取的初步研究[J]. 天然产物研究与开发, 2017, 29(增刊1): 113-118.