银条水苏糖提取条件研究

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (1): 123-130.

银条水苏糖提取条件研究

陈燕¹，钟先锋^1,2，黄桂东^1,2，李超波¹，常通¹，邓泽元^1*

¹南昌大学食品科学与技术国家重点实验室，南昌 330047；²河南科技大学食品科学和生物工程学院，洛阳 471003

收稿日期:2009-09-08 发布日期:2011-07-20
通讯作者: dengzy@ncu.edu.cn
基金资助:
教育部开放课题（NCU200509）

Extraction Conditions of Stachyose from Stachys floridana Schuttl. ex Benth

CHEN Yan¹, ZHONG Xian-feng^1,2, HUANG Gui-dong^1,2, LI Chao-bo¹, CHANG Tong¹, DENG Zen-yuan^1*

¹State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; ²Food Bioengineering College, Henan University of Science and Technology, Luoyang 471003, China

Received:2009-09-08 Published:2011-07-20

摘要/Abstract

摘要：

唇形科水苏属植物银条，也称银苗，药食两用，主产于偃师县（市），产量约有95%。本文以偃师产银条为原料，通过单因素、响应面分析法，研究了乙醇提取银条中水苏糖的最佳提取条件。以银条中水苏糖提取率为指标，通过单因素实验确定了银条水苏糖提取条件的参数范围，通过响应面分析法确定了其最佳提取条件。结果表明，最佳提取条件为乙醇浓度60%，液固比10:1，提取温度60 ℃，提取时间40 min，该条件下银条水苏糖提取率为46.8924%。

关键词: 银条, 水苏糖, 提取, 响应面

Abstract:

Stachys floridana schuttl. ex benth, also named yinmiao, a species of genus Stachys. And it is a special and traditional Chinese vegetable which can use as food and medicine. Yanshi city is the main origin with production over 95%. In this paper, the optimum extraction conditions of stachyose from yinmiao were studied by Response surface methodology (RSM). The results showed that the optimum extraction conditions for stachyose yield from yinmiao were as follows: extraction temperature 60 ℃, extraction time 40 min, ethanol concentration 60%, and ratio of liquid-solid 10:1. At these conditions, the yield of stachyose was 46.8924%. In conclusion, RSM was successfully applied to determine the optimum extraction conditions for stachyose from yinmiao.

Key words: Stachys floridana schuttl. Ex benth, stachyose, extraction, response surface methodology

陈燕，钟先锋，黄桂东，李超波，常通，邓泽元. 银条水苏糖提取条件研究[J]. 天然产物研究与开发, 2011, 23(1): 123-130.

CHEN Yan, ZHONG Xian-Feng, HUANG Gui-Dong, LI Chao-Bo, CHANG Tong, DENG Ze-Yuan. Extraction Conditions of Stachyose from Stachys floridana Schuttl. ex Benth[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(1): 123-130.

参考文献

1 Yin J,Yang G,Wang S,et al.Purification and determination of stachyose in Chinese artichoke (Stachys Sieboldii Miq.) by high-performance liquid chromatography with evaporative light scattering detection.Talanta,2006,70:208-212.
2 Dierking EC,Bilyeu KD.Raffinose and stachyose metabolism are not required for efficient soybean seed germination.J Plant Physiol,2009,166:1329-1335.
3 Muzquiz M,Burbano C,Pedrosa MM,et al.Lupins as a potential source of raffinose family oligosaccharides preparative method for their isolation and purification.Ind Crops Prod,1999,9(3): 183-188.
4 Pukacka S,Ratajczak E,Kalemba E.Non-reducing sugar levels in beech (Fagus sylvatica) seeds as related to withstanding desiccation and storage.J Plant Physiol,2009,2:1-10.
5 Csáky I,Fekete S.Soybean: feed quality and safety.Part 1:biologically active components.A review,Acta Vet Hung,2004,52: 299-313.
6 Zhang RX, Jia ZP, Kong LY,et al.Stachyose extract from Rehmannia glutinosa Libosch. to lower plasma glucose in normal and diabetic rats by oral administration.Pharmazie,2004,59:552-556.
7 Yin J,Yang G,Wang S,et al.Purification and determination of stachyose in Chinese artichoke (Stachys Sieboldii Miq.) by high-performance liquid chromatography with evaporative light scattering detection.Talanta,2006,70:208-212.
8 Rada V,Bartonová J,Vlková E.Specific growth rate of bifidobacteria cultured on different sugars.Folia Microbiol,2002,47: 477-480.
9 Liying Z,Li D,Qiao S,et al.Effects of stachyose on performance, diarrhoea incidence and intestinal bacteria in weanling pigs. Arch Tierernahr,2003,57(1):1-10.
10 Rada V,Nevoral J,Trojanová I,et al.Growth of infant faecal bifidobacteria and clostridia on prebiotic oligosaccharides in in vitro conditions.Anaerobe,2008,14:205-208.
11 Mei L,Wang ZC,Dai HJ,et al.Response surface optimization of polysaccharides extraction from liriope roots and its modulatory effect on sjogren syndrome.Int J Biol Macromol,2009,45:284-288.
12 Hao XC(郝学财),Yu XB(余晓斌),Liu ZY(刘志钰),et al.The appliaction of response surface methodolgy in optimization of micromialmedia.Nat Prod Res Dev(天然产物研究与开发),2006,27(1):38-41.

[1]	桂明安, 德兰, 杨贵斌, 高丹, 央卓玛, 卓玛东智, 袁瑞瑛. 藏药云南兔耳草总环烯醚萜苷闪式提取工艺优化及体外抗氧化、抗炎活性研究[J]. 天然产物研究与开发, 2024, 36(增刊1): 91-97.
[2]	张亚梅, 韩鸿萍, 宋道光. 响应面法优化暗紫贝母流浸膏提取工艺及其质量评价[J]. 天然产物研究与开发, 2024, 36(9): 1554-1563.
[3]	丁亚男, 王瑞阳, 高观祯, 汪惠勤, 周建武, 饶平凡. 油橄榄叶提取物对HaCaT细胞氧化损伤的保护作用及抑菌活性[J]. 天然产物研究与开发, 2024, 36(9): 1584-1591.
[4]	江丽洁, 温乐乐, 石佳艺, 邓雅慧, 刘巨钊, 袁强, 崔琦. 常山胡柚果皮精油提取工艺模型筛选优化、化学成分及抗氧化活性研究[J]. 天然产物研究与开发, 2024, 36(7): 1101-1111.
[5]	黄丽荣, 余佳, 李娇, 程莎骆衡. 基于网络药理学及实验验证探究苗药虎掌草不同溶剂提取物抗前列腺癌活性及其作用机制[J]. 天然产物研究与开发, 2024, 36(7): 1231-1241.
[6]	盛蒙珂, 赵莹菲, 田若丹, 文春南, 刘淼, 麻兵继. 怀山药内生真菌Chaetomium sp.的次生代谢产物研究[J]. 天然产物研究与开发, 2024, 36(6): 982-985.
[7]	李百元, 张叶敏, 夏晶晶, 姜红宇, 曹林艳, 陈丹. 19种中药提取物对海洋致病溶藻弧菌生长和生物被膜的影响[J]. 天然产物研究与开发, 2024, 36(6): 1013-1021.
[8]	宋钰, 纪丽莲, 刘艳丽, 王桃云. 枸骨的化学成分及其药理活性研究进展[J]. 天然产物研究与开发, 2024, 36(6): 1082-1099.
[9]	李超, 刘云华, 秦登云, 黄志芳, 刘玉红, 陈燕, 易进海. 基于正交设计优化新鲜川芎中挥发油提取工艺[J]. 天然产物研究与开发, 2024, 36(5): 771-778.
[10]	张宏岐, 朱烈琴, 曾亚妮, 崔敏, 周莹霞, 杜娟. 忍冬木层孔菌多糖的提取工艺优化及其体外抗氧化活性研究[J]. 天然产物研究与开发, 2024, 36(4): 581-588.
[11]	邓超凡, 江志波, 马晓莉, 南泽东. 紫堇属植物苄基异喹啉类成分研究进展[J]. 天然产物研究与开发, 2024, 36(4): 694-716.
[12]	张一宁, 于志琪, 屈施旭, 刘同歌, 张玉红. 火麻叶中大麻二酚提取工艺的响应面法优化及不同部位含量分布[J]. 天然产物研究与开发, 2024, 36(3): 369-376.
[13]	邵林子, 程玉娇, 黄林华, 谈安群, 王华. 超声辅助深共晶溶剂高效提取枳实辛弗林的工艺优化及提取动力学分析[J]. 天然产物研究与开发, 2024, 36(3): 499-511.
[14]	宋丽娅, 李丽华, 毕思童. 基于网络药理学、分子对接技术及实验验证研究黄芪提取物治疗缺血缺氧性脑病的机制[J]. 天然产物研究与开发, 2024, 36(3): 520-527.
[15]	张闻婷, 杨旻静, 梅瑜, 王继华. 金线莲苷的研究现状[J]. 天然产物研究与开发, 2024, 36(2): 348-356.