新疆胀果甘草总黄酮的细胞毒活性研究

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (1): 49-53.

新疆胀果甘草总黄酮的细胞毒活性研究

郭霞¹，盛磊¹，木合布力•阿布力孜²，阿布力孜•阿布杜拉^3*

¹新疆医科大学，新疆地方病分子生物学重点实验室；²新疆医科大学药学院药化有机教研室； ³新疆医科大学基础医学院生物化学暨分子生物学教研室，乌鲁木齐830011

收稿日期:2009-08-10 发布日期:2011-07-20
通讯作者: abulizi_de@126.com
基金资助:
新疆维吾尔自治区重点实验室基金（XJDX0208-2006-02）

Cytotoxic Activity of Flavonoids from Xinjiang Glycyrrhiza Inflata Bat.

GUO Xia¹, SHENG Lei¹, MOURBOUL Ablise², Abulizi ABUDULA^3*

¹Xinjiang Key Laboratory of Molecular Biology and Endemic Diseases; ²Department of Pharmaco-chemistry; ³Department of Biochemistry and Biology, Basic Medical College, Xinjiang Medical University, Urumqi 830011, China

Received:2009-08-10 Published:2011-07-20

摘要/Abstract

摘要：

为了探讨新疆胀果甘草总黄酮（general flavonoids, GFs）对宫颈癌细胞的细胞毒活性，自制备胀果甘草GFs，通过离子阱喷雾式质谱技术分析，建立GFs组分的指纹图谱；并且对SiHa宫颈癌细胞进行GFs药物干预，应用MTT法和流式细胞技术分别鉴定细胞活力和细胞凋亡率。获得了新疆胀果甘草GFs组分的特征性指纹图谱，提取物中GFs含量可达35.40%；发现在0~500 μg/mL GFs浓度范围内，GFs药物干预引起细胞活力梯度性下降，其MTT法检出的最低值为12%；在0~1000 μg/mL GFs浓度范围内，GFs诱导的细胞凋亡率呈现梯度性上升趋势，其流式细胞仪检出的最高值为78%。结果表明新疆胀果甘草GFs具有较强的细胞毒活性，能够抑制宫颈癌细胞生长与活力，并诱导细胞凋亡。

关键词: 新疆胀果甘草, 总黄酮, 细胞毒活性

Abstract:

To investigate the cytotoxic activity of general flavonoids (GFs) from Xinjiang Glycyrrhiza inflata Bat, on cervical carcinoma cells. GFs was prepared from Glycyrrhiza inflata, and the finger print map was characterized by Electro Spray Mass Spectrometry; SiHa cervical carcinoma cells were treated with GFs, and the cell viability and cellular apoptosis were determined by MTT method and flow cytometry, respectively. We discovered that GFs from Xinjiang Glycyrrhiza inflata had special finger print characteristics; the content of flavonoids in the extract was 35.40%; in the dose range of 0-500 μg/mL GFs, the cell viability dropped gradually with the GFs treatment, and the lowest level by MTT detection was 12%; in the dose range of 0-1000 μg/mL GFs, the ratio of cellular apoptosis induced by GFs increased gradually, and the highest level by flow cytometry detection was up to 78%. GFs from Xinjiang Glycyrrhiza inflata had relatively high cytotoxic activity including the inhibition of cell growth and viability as well as the induction of cellular apoptosis. In conclusion, this work may provide important evidence for further screening and isolation of active ingredients of flavonoids as well as the development of new natural anti-cancer drugs.

Key words: Xinjiang Glycyrrhiza inflata, general flavonoids (GFs), cytotoxic activity

中图分类号:

R931.6 Q949.751.9

郭霞，盛磊，木合布力?阿布力孜，阿布力孜?阿布杜拉. 新疆胀果甘草总黄酮的细胞毒活性研究[J]. 天然产物研究与开发, 2011, 23(1): 49-53.

GUO Xia, SHENG Lei, MU He-Bu-Li-a?￠A-Bu-Li-Zi, A Bu-Li-Zi-a?￠A-Bu-Du-La. Cytotoxic Activity of Flavonoids from Xinjiang Glycyrrhiza Inflata Bat.[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(1): 49-53.

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