三棱黄酮体外诱导A549及MCF-7细胞S/G2周期停滞的研究

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (2): 224-227.

三棱黄酮体外诱导A549及MCF-7细胞S/G₂周期停滞的研究

孙杰，王芍，郭斌，尉亚辉^*

西北大学生命科学学院西部资源生物与现代生物技术教育部重点实验室，西安 710069

收稿日期:2010-03-26 出版日期:2011-04-25 发布日期:2011-10-14
通讯作者: weiyahui@nwu.edu.cn
基金资助:
国家自然科学基金（31000144），陕西省教育厅专项（09JK746），“十一五”国家科技支撑计划项目课题子课题（2006BAD06B00）

Flavonoids of Rhizoma Sparaganii Induce S/G₂ Stage Arrest in A549 and MCF-7 Cells

孙杰，王芍，郭斌，尉亚辉^*

Key laboratory of Resource Biology and Biotechnology in Western China, College of Life Science, Northwest University, Xi'an 710069, China

Received:2010-03-26 Online:2011-04-25 Published:2011-10-14

摘要/Abstract

摘要：

为了研究三棱黄酮（Rhizoma Sparganii flavonoids，RSF）对雌激素受体阳性（estrogen receptor positive，ER⁺）恶性肿瘤细胞株A549与MCF-7的细胞毒作用和机制，本次试验制备含188 µg/mL、375 µg/mL、750 µg/mL RSF的DMEM培养基（RSFD-188、RSFD-375、RSFD-750），与贴壁后的A549及MCF-7细胞共培养3 d，含1%甲醇的DMEM培养基为对照，使用MTT法、免疫荧光技术、流式细胞术比较组间细胞的增殖活性、形态（anti-a-tublin）、凋亡率以及细胞周期的差异。试验结果显示，RSF可呈剂量性抑制A549与MCF-7细胞的增殖活性、增加凋亡小体比率、诱导细胞骨架形态异常改变；与RSFD-375、RSFD-750共培养的A549与MCF-7细胞表现为明显的S/G₂细胞周期停滞。所得实验结果表明，RSF对A549及MCF-7细胞具有明确的细胞毒理作用，其抑制细胞增殖活性的机制与诱导细胞的S/G2细胞周期停滞相关。

关键词: 三棱, 黄酮苷, 雌激素受体阳性, A549, MCF-7

Abstract:

To investigate the cytotoxicity effects and mechanisms on estrogen receptor positive (ER⁺) cancer cell lines A549 and MCF-7 of the Rhizoma Sparganii flavonoids (RSF)，A549 or MCF-7 cells in DMEM mediums with 188，375，and 750 µg/mL RSF were cultured for 3 days，respectively. DMEM with 1% methanol culture cells as control. The cell proliferation activity，anti-a-tublin form，apoptosis as well as cell cycle differences between groups were studied by MTT，immunofluorescence，and flow cytometry technology. As a result，compare to the 1% methanol control groups，RSF could inhibit the proliferation activity，increase the rate of apoptotic bodies，and induce cytoskeleton disorder of the A549 and MCF-7 cells with the dose-related. In addition，S/G₂stage arrests were induced obviously in A549 and MCF-7 cells cultured with 375 and 750 µg/mL RSF. The results indicated that RSF showed a notable cytotoxicity against A549 and MCF-7 cells，and the inhibitions of cell proliferation were related with S/G2 cell cycle arrests.

Key words: Rhizoma Sparganii, flavonoid glycosides, estrogen receptor positive, A549, MCF-7

孙杰，王芍，郭斌，尉亚辉. 三棱黄酮体外诱导A549及MCF-7细胞S/G₂周期停滞的研究[J]. 天然产物研究与开发, 2011, 23(2): 224-227.

SUN Jie, WANG Shao, GUO Bin, WEI Ya-Hui. Flavonoids of Rhizoma Sparaganii Induce S/G₂ Stage Arrest in A549 and MCF-7 Cells[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(2): 224-227.

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三棱黄酮体外诱导A549及MCF-7细胞S/G₂周期停滞的研究

Flavonoids of Rhizoma Sparaganii Induce S/G₂ Stage Arrest in A549 and MCF-7 Cells

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