海洋链霉菌S007次生代谢产物的分离鉴定（英文）

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (3): 410-414.

海洋链霉菌S007次生代谢产物的分离鉴定（英文）

张宏宇^1,2，秦梅³，李富超⁴，LAATSCH Hartmut⁵，王宏鹏⁵，秦松^1*

¹中国科学院烟台海岸带研究所，烟台 264003；²中国科学院研究生院，北京 100049；³曲阜师范大学化学与化工学院，曲阜 273165；⁴中国科学院海洋研究所，青岛 266071；⁵Department of Organic and Biomolecular Chemistry,University of Göttingen,Göttingen,D-37077,Germany

收稿日期:2010-07-08 出版日期:2011-06-25 发布日期:2011-08-08
通讯作者: sqin@yic.ac.cn

Isolation and Identification of Metabolites Produced by Marine Streptomyces sp.S007

ZHANG Hong-yu^1,2, QIN Mei³, LI Fu-chao⁴, LAATSCH Hartmut⁵, WANG Hong-peng⁵, QIN Song^1*

¹Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; ²Graduate University of Chinese Academy of Sciences, Beijing 100049, China; ³School of chemistry and chemical engineering, Qufu Normal University, Qufu 273165, China; ⁴Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; ⁵Department of Organic and Biomolecular Chemistry, University of Göttingen, Göttingen, D-37077, Germany

Received:2010-07-08 Online:2011-06-25 Published:2011-08-08
Supported by:
This work was supported by the International Innovation Partnership Program: Typical Environmental Process and Effects on Resources in Coastal Zone Area, the research fund of Qufu Normal University (XJ0718),the National Natural Science Foundation of China (31000037), and the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-YW-G-073, KZCX2-YW-216, KZCX2-YW-209)

摘要/Abstract

摘要：

采用柱层析、制备薄层层析等方法从海洋链霉菌S007的发酵液提取物中分离得到了9个化合物，经波谱分析确定为嘧啶（1），吡咯-2-羧酸（2），卡拉霉素（3），3-吲哚丙酸（4），吲哚-3-羧酸（5），N-乙酰基酪胺（6），2'-胸腺嘧啶脱氧核苷（7），N-β-乙酰色胺酸（8）和三乙胺盐酸盐（9）。其中三乙胺盐酸盐是首次从链霉菌中得到；海虾致死实验结果显示：化合物3在40 µg/mL浓度下对丰年虾的致死率为86.5%，表明卡拉霉素对丰年虾表现出很强的毒性作用。

关键词: 海洋链霉菌S007, 次生代谢产物, 海虾, 细胞毒性, 卡拉霉素

Abstract:

Nine metabolites thymine (1), pyrrole-2-carboxylic acid (2), kalamycin (3), 3-indole propionic acid (4), indol-3-carboxylic acid (5), N-acetyl-tyramine (6), 2'-deoxythymidine (7), N-β-acetyltryptamine (8), and triethylamine hydrochloride (9) were isolated by column and preparative thin layer chromatography from the extract of fermented broth of Streptomycete sp. S007. Their structures were established by NMR and MS data. Among of them, 9 was firstly obtained from Streptomycete; The brine shrimp bioassay showed the mortality rates of kalamycin to brine shrimp are 86. 5% at a concentration of 40 µg/mL, indicating kalamycin possessed significant toxicity on brine shrimp.

中图分类号:

R284.2

张宏宇，秦梅，李富超，LAATSCH Hartmut，王宏鹏，秦松. 海洋链霉菌S007次生代谢产物的分离鉴定（英文）[J]. 天然产物研究与开发, 2011, 23(3): 410-414.

ZHANG Hong-yu, QIN Mei, LI Fu-chao, LAATSCH Hartmut, WANG Hong-peng, QIN Song. Isolation and Identification of Metabolites Produced by Marine Streptomyces sp.S007[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 410-414.

参考文献 12

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