AB-8型树脂对无患子皂苷的动态吸附与解吸性能

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (4): 709-712.

AB-8型树脂对无患子皂苷的动态吸附与解吸性能

康家胜，方春，张静，魏凤玉*

合肥工业大学化工学院，合肥 230009

收稿日期:2010-02-04 出版日期:2011-08-25 发布日期:2011-09-13
基金资助:
安徽高校省级自然科学研究重点项目（KJ2009A002），合肥工业大学研究生创新基金项目(XS08042)

Dynamic Adsorption and Desorption of Sapindus-saponins onto AB-8 Resin

KANG Jia-sheng, FANG Chun, ZHANG Jing, WEI Feng-yu*

School of Chemical Engineering, Hefei University of Technology, Hefei 230009, China

Received:2010-02-04 Online:2011-08-25 Published:2011-09-13

摘要/Abstract

摘要： 采用单因素实验法，以树脂的饱和吸附量、产品的得率及纯度为指标，考察各因素对动态吸附与解吸的影响，优化大孔树脂动态吸附分离无患子皂苷的工艺条件。结果表明，当pH为4.5的无患子皂苷水提液以流速1 mL/min通过高径比为5.4:1的吸附柱时，AB-8树脂对无患子皂苷的饱和吸附量达568 mg/g；采用1.5 BV的95%乙醇以1 mL/min的流速洗脱吸附后的树脂，产品得率为83.48%、纯度达93.00%；树脂重复使用8次后其吸附解吸性能基本不变。该方法可以用于无患子皂苷的工业化分离提纯。

关键词: 无患子皂苷；AB-8树脂；动态吸附-解吸, 吸附量

Abstract: To optimize the dynamic adsorption and desorption process parameters for sapindus-saponins onto AB-8 resin, the monofactorial experiments were used to analyze the effects of the process parameters on the saturated adsorption capacity, the recovery yield and the purity of the sapindus-saponins. The results showed that the saturation adsorption capacity of sapindus-saponins on AB-8 resin achieved 568 mg/g when the crude sapindus- saponins water extracts (pH4.5) ?owed through the resin column (the ratio of height to diameter 5.4:1) at 1 mL/min. The elution parameters were as follows: 1.5 BV 95% ethanol aqueous solutions at 1 mL/min. The sapindus-saponins content in the ?nal product was 93% with a recovery yield of 83.48%. In addition, the stability of AB-8 resin was fine after eight-time recycled. This method could provide a potential approach for large-scale separation and puri?cation of sapindus-saponins for its wide pharmaceutical use.

Key words: sapindus-saponins, AB-8 resin, dynamic adsorption-desorption, adsorption capacity

中图分类号:

TQ028.1+5

康家胜，方春，张静，魏凤玉. AB-8型树脂对无患子皂苷的动态吸附与解吸性能[J]. 天然产物研究与开发, 2011, 23(4): 709-712.

KANG Jia-sheng, FANG Chun, ZHANG Jing, WEI Feng-yu. Dynamic Adsorption and Desorption of Sapindus-saponins onto AB-8 Resin[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(4): 709-712.