猪苓及猪苓多糖对BBN联合糖精作用Fisher-344大鼠肝脏代谢酶的影响

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (5): 923-926.

猪苓及猪苓多糖对BBN联合糖精作用Fisher-344大鼠肝脏代谢酶的影响

张国伟1，李彩霞2，王艳峰3，黄羽2，苏芝军4，郑芳2，曾星2*

1河北大学中医学院，保定 071002；2广州中医药大学第二临床医学院，广州 510006；3中国医药集团四川抗菌素工业研究所，成都 610051；4陕西省府谷县人民医院，府谷719400

收稿日期:2010-08-23 出版日期:2011-10-25 发布日期:2011-09-26
基金资助:
广东省科技计划项目（2010B030700059）；河北大学科研基金资助项目（2101Q46）；河北省卫生厅科研基金项目（20110514）

Effects of Aqueous Extract of Sclerotia of Polyporus umbellatus and Polyporus Polysaccharide on Liver Metabolic Enzyme of Fisher-344 Rats Induced by BBN Combined Saccharin

ZHANG Guo-wei1,LI Cai-xia2,WANG Yan-feng 3,HUANG Yu2,SU Zhi-jun4,ZHENG Fang2,ZENG Xing2*

1College of Chinese Medicine,Hebei University,Baoding 071002,China; 2Guangdong Provincial Academy of Chinese Medical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China; 3Sinopharm ChuanKong pharmaceutical CO.,LTD,Chengdu 610051,China; 4Fugu Peoples Hospital of Shanxi,Fugu 719400,China

Received:2010-08-23 Online:2011-10-25 Published:2011-09-26

摘要/Abstract

摘要： 以BBN联合糖精作用Fisher-344大鼠，分别使用ELISA及RT-PCR方法测定样本中CYP450、GST和NQO1酶活性及Gstpi和NQO1 mRNA相对表达含量。结果表明，猪苓及猪苓多糖均可使样本中CYP450酶活性显著降低，其中猪苓对CYP450酶活性抑制呈剂量依赖性；猪苓及猪苓多糖对样本中GST无明显影响，猪苓可显著升高样本中NQO1酶活性；猪苓高剂量及猪苓多糖可上调GSTPi mRNA表达，猪苓及猪苓多糖对NQO1 mRNA表达无明显作用。研究表明猪苓及猪苓多糖抗癌作用与降低CYP450与升高NQO1酶活性有关，但机制有待进一步研究。

关键词: 猪苓, 猪苓多糖, 细胞色素P450, Ⅱ相代谢酶

Abstract: The present aimed to evaluate effects of sclerotia of P.umbellatus FRIES aqueous extract (SPUE) and Polyporus polysaccharide (PPS) on liver metabolic enzyme of Fisher-344 rat induced by N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) combined saccharin.The rat liver enzyme activities of CYP450,glutathione S-transferase (GST),and NAD(P)H:quinone oxidoreductase 1 (NQO1) were determined by ELISA.The relative expressions of liver glutathione S-transferase π (GSTPi) and NQO1 mRNA were measured with RT-PCR.The present report showed the SPUE and PPS decreased the activity of rat liver CYP450 enzyme，the effect had dose-effect relationship; they had no significant effect on the activity of rat liver GST enzyme,and they could increase the activity of rat liver NQO1 enzyme; the high dose SPUE could up-regulate the GSTPi mRNA relative expression in rat liver,they had no effect on the NQO1 mRNA relative expression in rat liver.In conclusion,the inhibition of bladder carcinogenesis by SPUE and PPS associate with the decreasing of rat liver CYP450 enzyme activity and increasing of NQO1 enzyme activity,but the mechanism needs further research.

Key words: sclerotia of Polyporus umbellatus, Polyporus polysaccharide, CYP450, Ⅱ phase enzyme

中图分类号:

R285.5

张国伟，李彩霞，王艳峰，黄羽，苏芝军，郑芳，曾星. 猪苓及猪苓多糖对BBN联合糖精作用Fisher-344大鼠肝脏代谢酶的影响[J]. 天然产物研究与开发, 2011, 23(5): 923-926.

ZHANG Guo-wei, LI Cai-xia, WANG Yan-feng, HUANG Yu, SU Zhi-jun, ZHENG Fang, ZENG Xing. Effects of Aqueous Extract of Sclerotia of Polyporus umbellatus and Polyporus Polysaccharide on Liver Metabolic Enzyme of Fisher-344 Rats Induced by BBN Combined Saccharin[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(5): 923-926.

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