关键词: 放线菌, 稻瘟霉, 抗肿瘤活性, 肝癌细胞BEL-7402, 胃癌细胞BGC-823

Abstract: An anti-tumour strain JXJ-0108 was screened out from 150 actinomycetes strains by detecting deformation of mycelia of Pyricularia oryzae, and compound JXJ-0108-1, which showed strong anti-tumour activity and taked on potential value in the development of anti-gastric cancer and hepatoma drugs, was purified from the fermentation liquid. The antitumor activity of the compound was examined by MTT( Microculture Tetrozolium) method in vitro, and the IC₅₀ values on human hepatic carcinoma cells BEL-7402 and gastric carcinoma cells BGC-823 were 1.63 and 0.95 ug/mL, respectively. Compared with the control groups, the proportions of hepatic carcinoma cells BEL-7402 and gastric carcinoma cells BGC-823 in G0/G1, S and G2/M phases presented very notable difference after treated with the compound from the concentrations of 0.50 and 0.30 ug/mL, respectively. As the results, the proportions of cells in G0/G1 and S phases were reduced, while the proportion of cells in G2/M phase was increased. This paper provides a quick and convenient method to screen anti-tumour actinomycetes and track the active compounds during the purification efficaciously.

Key words: actinomycete, Pyricularia oryzae, anti-tumour activities, hepatic carcinoma cells BEL-7402, gastric carcinoma cells BGC-823