天然产物研究与开发 ›› 2012, Vol. 23 ›› Issue (2): 212-215.doi: A

• 开发研究 • 上一篇    下一篇

微生物发酵炮制何首乌机理的初步研究

杜晨晖1，闫 艳1，原红霞1，赵荣华2*，赵声兰2，王兴红3   

  1. 1山西中医学院，太原 030024；2云南中医学院中药学院，昆明6505002；3云南大学云南省微生物研究所，昆明650091
  • 收稿日期:2011-09-01 出版日期:2012-02-29 发布日期:2012-03-23
  • 基金资助:

    国家自然科学基金项目（30760312）

Preliminary Study on the Processing Mechanism of Polygonum multiflorum Thunb by Microbial Fermentation

DU Chen-hui1, YAN Yan1, YUAN Hong-xia1, ZHAO Rong-hua2*, ZHAO Sheng-lan2, WANG Xing-hong3   

  1. 1Shanxi College of Traditional Chinese Medicine, Taiyuan 030024, China; 2College of Traditional Chinese Pharmacy, Yunnan College of Traditional Chinese Medicine, Kunming 650500, China; 3Yunnan University, Microbe Research Institute, Kunming 650091, China
  • Received:2011-09-01 Online:2012-02-29 Published:2012-03-23

摘要： 为探讨微生物发酵炮制何首乌的机制，采用HPLC考察何首乌微生物发酵前后二苯乙烯苷类和蒽醌类化学成分的变化。何首乌经米根霉发酵后，产生了新的蒽醌类成分大黄素-6-O-β-D-吡喃葡萄糖苷，而二苯乙烯苷类成分无变化。同时，药理研究发现，大黄素-6-O-β-D-吡喃葡萄糖苷对家兔肠平滑肌的收缩作用弱于大黄素。由此推断，在何首乌发酵炮制过程中，米根霉可诱导大黄素转化为大黄素-6-O-β-D-吡喃葡萄糖苷，从而降低何首乌的泻下作用。实验结果初步验证了微生物发酵炮制何首乌的科学性。

关键词: 微生物发酵；何首乌；大黄素；大黄素-6-O-&beta, -D-吡喃葡萄糖苷；兔离体回肠

Abstract: The aim of this study was to investigate the processing mechanism of Polygonum multiflorum Thunb by microbial fermentation. The anthraquinones and di-phenyl ethylene glycoside in fermented and no-fermented samples were determined by HPLC, and emodin-6-O-?-D-glucopyranoside was produced in P. multiflorum after fermentation. However, no evident change was identified for di-phenyl ethylene glycoside. In addition, the contraction action of the emodin-6-O-?-D-glucopyranoside was weaker than that of emodin on excised rabbit ileum, which suggested that the purgative activity of P. multiflorum was reduced after fermentation. The results preliminarily indicate that the processing for P. multiflorum by microbial fermentation is scientific.

Key words: microorganism fermentation, Polygonum multiflorum Thunb, emodin, emodin-6-O-?-D-glucopyranoside, excised rabbit ileum

中图分类号: 

R282.71 Q939.1

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