油菜蜂花粉超临界提取物HPLC-FLD分析及体外清除自由基活性研究

天然产物研究与开发 ›› 2013, Vol. 25 ›› Issue (12): 1631-1637.

油菜蜂花粉超临界提取物HPLC-FLD分析及体外清除自由基活性研究

吕欢欢1,2，王小艳1*，王洪伦1，索有瑞1

1 中国科学院西北高原生物研究所，西宁 810001；2 中国科学院大学，北京 100049

出版日期:2013-12-31 发布日期:2015-01-08

Analysis of Rape Bee Pollen Supercritical Fluid Extract Using HPLC-FLD and Its Free Radicals Scavenging Activity Investigation

LV Huan-huan1,2, WANG Xiao-yan1*, WANG Hong-lun1, SUO You-rui1

1 Northwest Institution of Plateau Biology,Chinese Academy of Sciences,Xining,810001,China; 2 University of Chinese Academy of Sciences,Beijing 100049,China

Online:2013-12-31 Published:2015-01-08

摘要/Abstract

摘要： 本实验通过柱前荧光衍生HPLCFLD对油菜蜂花粉超临界提取物（Rape bee pollen supercritical fluid extract，PSFE）中的主要成分进行了定性及定量分析，并采用超氧阴离子自由基（O₂^-·）体系、二苯代苦味酰基自由基（DPPH·）体系和羟基自由基（·OH）体系考察了PSFE提取物的体外清除自由基活性。结果表明，PSFE中含有丰富的游离脂肪酸，其中饱和脂肪酸占总游离脂肪酸的58%，不饱和脂肪酸占42%，尤其是不饱和脂肪酸C18:3的含量最高，达到了10933.69 μg/g。同时，PSFE对O₂^-·、DPPH·和·OH这三种典型的自由基均具有不同程度的清除作用，其中对DPPH·的清除能力高于阳性对照物L-抗坏血酸，并且随浓度的增大清除效果更显著，最高可达60.29%；而对O₂^-·和·OH的清除效果在实验浓度范围内最高可分别为11.99%和7.59%，比阳性对照物低。

关键词: 油菜蜂花粉超临界提取物, 柱前荧光衍生, HPLC-FLD, 超氧阴离子自由基, 羟基自由基, 二苯代苦味酰基自由基

Abstract: In the present study,the main components of rape bee pollen supercritical fluid extract (PSFE) were qualitatively and quantitatively analyzed by HPLC with fluorescence detector (FLD) using pre-column fluorescence derivatization.In addition,superoxide anion free radical (O₂^-·),DPPH free radical (DPPH·) and hydroxyl free radical (·OH) assays were used to evaluate the free radicals scavenging activities of PSFE.The results indicated that PSFE contained abundant free fatty acid (FFA),in which the saturated FFA accounted for 58% of the total FFA and the unsaturated FFA accounted for 42%.The unsaturated FFA C18:3 was determined to have the highest concentration (10933.69 μg/g).Moreover,PSFE showed various degrees of ability to scavenge these three typical free radicals.The scavenging effects of PSFE on DPPH· were much higher than the positive control L-ascorbic acid.As the concentration increased,the scavenging rate was getting more significant,even reach to 60.29%.Nevertheless,the scavenging rates of PSFE on O₂^-·and ·OH were both less significant than the positive control and were 11.99% and 7.59%,respectively,in the range of experimental concentrations.

Key words: rape bee pollen supercritical fluid extract, pre-column fluorescence derivatization, HPLC-FLD, superoxide anion free radical, DPPH free radical, hydroxyl free radical

中图分类号:

TS202.3

吕欢欢1,2，王小艳1*，王洪伦1，索有瑞1. 油菜蜂花粉超临界提取物HPLC-FLD分析及体外清除自由基活性研究[J]. 天然产物研究与开发, 2013, 25(12): 1631-1637.

LV Huan-huan1,2, WANG Xiao-yan1*, WANG Hong-lun1, SUO You-rui1. Analysis of Rape Bee Pollen Supercritical Fluid Extract Using HPLC-FLD and Its Free Radicals Scavenging Activity Investigation[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2013, 25(12): 1631-1637.

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