黄花蒿青蒿酸制备及其生物稳定性研究

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (10): 1557-1561.

黄花蒿青蒿酸制备及其生物稳定性研究

徐定华1,2，唐雯熙1,2，张晓蓉1,2*，李杰2，刘继旋2

1吉首大学植物资源保护与利用湖南省高校重点实验室；2吉首大学生物资源与环境科学学院，湖南吉首 416000

出版日期:2014-10-30 发布日期:2014-10-31

Preparation of Artemisinic Acid from Artemisia annua L.and Its Biological Stability

XU Ding-hua1,2, TANG Wen-xi1,2, ZHANG Xiao-rong1,2*, LI Jie2, LIU Ji-xuan2

1 Key Laboratory of Plant Resources Conservation and Utilization in Hunan Province,Jishou University; 2 College of Bioresources and Environment Science,Jishou University,Jishou 416000, China

Online:2014-10-30 Published:2014-10-31

摘要/Abstract

摘要： 基于青蒿酸重要的药用和开发价值，采用溶剂法、吸附分离法从黄花蒿植物制备青蒿酸，采用光谱、质谱和熔点法表征青蒿酸晶体，采用HPLC法分析其生物稳定性。通过制备工艺获得纯度为96%的青蒿酸结晶，产率为61.7%。4 ℃、室温、60 ℃以及自然光照条件下保存30 d青蒿酸具有较好的生物稳定性；植物体内外青蒿酸均能稳定保存一年，含量基本不变。紫外光照条件下青蒿酸易光解，6 h后基本检测不出青蒿酸。通过制备工艺获得高纯度的青蒿酸结晶，青蒿酸对温度和自然光照较为稳定，在植物体内外均可保存一年。紫外光对青蒿酸具有较强的破坏作用。

关键词: 黄花蒿, 青蒿酸, 制备工艺, 生物稳定性

Abstract: In this paper,the preparation of artemisinic acid from Artemisia annua L.was investigated and its biological stability was explored.Liquidliquid extraction method and adsorptive separation method were used to prepare artemisinic acid from A.annua,and spectroscopic,mass spectrometry and melting point method were adopted to characterize artemisinic acid crystals.The biological stability of artemisinic acid was analyzed using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC).Artemisinic acid crystal was successfully obtained by liquidliquid extraction method and Adsorptive separation method,and the yield of product reached to 61.7% and the purity of product was higher than 96%.Artemisinic acid was very stable under the conditions of 4 oC,room temperature,60 ^oC and natural lighting for 30 d.Furthermore,it was stably preserved in plant for a year with its content maintained at the same level.The structure of product was badly destructed by UV light,and it was not detected after lighting for 6 h.In conclusion,high purity of artemisinic acid could be obtained by the developed preparation methods.It was more stable at the conditions of natural light and temperature,and the artemisinic acid could save one year in vivo and in vitro. However,UV light strongly destroy artemisinic acid.

Key words: Artemisia annua L., artemisinic acid;preparation technology;biological stability

中图分类号:

R284.6

徐定华，唐雯熙，张晓蓉，李杰，刘继旋. 黄花蒿青蒿酸制备及其生物稳定性研究[J]. 天然产物研究与开发, 2014, 26(10): 1557-1561.

XU Ding-hua1,2, TANG Wen-xi1,2, ZHANG Xiao-rong1,2*, LI Jie2, LIU Ji-xuan2. Preparation of Artemisinic Acid from Artemisia annua L.and Its Biological Stability[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(10): 1557-1561.

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