天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (10): 1562-1567.

• 研究论文 • 上一篇    下一篇

大孔树脂分离纯化陈皮中多甲氧基黄酮类化合物

刘韶1*,阳秀娟 2   

  1. 1中南大学湘雅医院药学部,湖南长沙 410008;2桂林医学院附属医院临桂院区药剂科,广西桂林541100
  • 出版日期:2014-10-30 发布日期:2014-10-31

Separation and Purification of Polymethoxylated Flavones from Orange Peel Using Macroporous Resin

LIU Shao1,YANG Xiu-juan2   

  1. 1Department of pharmacy,Xiangya Hospital,Central south University,Hunan Changsha 410008,China; 2 Department of pharmacy,Guilin Hospital of Medical University and Lingui branch,Guangxi Guilin 541100,China
  • Online:2014-10-30 Published:2014-10-31

摘要: 以川陈皮素和橘皮素为评价指标,筛选陈皮中多甲氧基黄酮类的大孔树脂纯化工艺。采用高效液相色谱法检测川陈皮素和橘皮素;采用静态和动态吸附、解吸实验筛选大孔树脂种类和工艺参数。D101型大孔树脂对陈皮中多甲氧基黄酮类吸附最好,最佳工艺条件为:洗脱剂为80%乙醇,洗脱剂用量为6倍柱体积(BV),最佳上样液浓度为500 mg/mL;经过处理后川陈皮素和橘皮素的纯度分别提高了3.7倍和3.2倍。大孔树脂能用于陈皮中多甲氧基黄酮类化合物的分离纯化。

关键词: 陈皮, 大孔树脂, 多甲氧基黄酮, 川陈皮素, 橘皮素

Abstract: The enrichment process of polymethoxylated flavones from dried tangerine or orange peel using macroporous resin were optimized.Based on the contents of nobiletin and tangeretin determined by HPLC,the adsorption capacity and elution ratio of different resins were studied and compared.D101 macroporous resin was most suitable for separation and purification of polymethoxylated flavones from orange peel.The concentration and dosage of eluent,sample concentration were investigated and the optimal conditions were obtained as follows:the eluent was 80% ethanol,dosage of eluent was 6 BV,sample concentration was 500 mg/mL.After processing,the purities of nobiletin and tangeretin were improved 3.7 times and 3.2 times,respectively.The results proved that macroporous resin can be used for separation and purification of polymethoxylated flavones from orange peel.

Key words: orange peel, macroporous resin, polymethoxylated flavones, nobiletin, tangeretin

中图分类号: 

R284