天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (10): 1680-1684.

• 开发研究 • 上一篇    下一篇

N-琥珀酰低聚壳聚糖的抗氧化性能研究

李铭,熊小英,谢晶,薛斌,银旭红,孙涛*   

  1. 上海海洋大学食品学院 上海水产品加工储藏工程研究中心,上海 201306
  • 出版日期:2014-10-30 发布日期:2014-10-31

Antioxidant Activity of N-Succinyl Chitosan Oligosaccharides

LI Ming,XIONG Xiao-ying,XIE Jing,XUE Bin,YIN Xu-hong,SUN Tao*   

  1. College of Food Science & Technology,Shanghai Ocean University,Shanghai Engineering Research Center of Aquatic Product Processing & Preservation, Shanghai 201306, China
  • Online:2014-10-30 Published:2014-10-31

摘要: 低聚壳聚糖经化学改性得到三种取代度不同的N-琥珀酰低聚壳聚糖(NSCOSA 、NSCOSB和NSCOSC),取代度(DS)分别是为0.25、0.57和0.70。对其结构进行红外表征,并且并考察了其对超氧阴离子自由基(O2·)、羟基自由基(·OH)、DPPH的清除活性以及还原能力。结果表明:N-琥珀酰低聚壳聚糖衍生物的抗氧化活性与低聚壳聚糖相比有所降低。随着DS的升高,琥珀酰衍生物对·OH的清除能力下降;对O2·的清除能力大小顺序为NSCB>NSCA>NSCC;对DPPH的清除能力以及还原能力大小顺序均为:NSCA>NSCC>NSCB。这可能是由于N-琥珀酰低聚壳聚糖的取代度、引进基团的性质以及对自由基的清除机理不同所致。

关键词: 低聚壳聚糖, N-琥珀酰低聚壳聚糖, 抗氧化活性

Abstract: Three N-succinyl chitosan oligosaccharides (NSCOSs) with different substituting degrees (NSCOSA:0.25,NSCOSB:0.57 and NSCOSC:0.70,respectively) were prepared by reacting of chitosan oligosaccharide (COS) and succinic anhydride.The structure changes of the COS derivatives were characterized by Fourier transform infrared (FT-IR) spectra.Their antioxidant activities were evaluated by the scavenging of superoxide radical O2·,hydroxyl radical ·OH, 1,1-diphenyl-2-picrylhrazyl radical (DPPH) and determination of reducing power.The results indicated that all the NSCOSs showed obvious antioxidant activity,the orders of their scavenging effect on O2· was NSCOSB>NSCOSA>NSCOSC,the scavenging effect on ·OH decreased with the increasing of substituting degrees (DSs), the orders of their scavenging effect on DPPH and reducing power both were:NSCOSA>NSCOSC>NSCOSB.The result may be related with the substituting degrees,the properties of the substituting group and the different scavenging mechanisms against free radicals.

Key words: chitosan oligosaccharide, N-succinyl chitosan oligosaccharide, antioxidant activity

中图分类号: 

R284.2