汉麻提取物的抗紫外功效筛选和安全性评价

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (11): 1872-1878.

汉麻提取物的抗紫外功效筛选和安全性评价

何聪芬1*，李靖宇2，王领3，何锦风4

1 北京工商大学北京市植物资源研究开发重点实验室；2 首都师范大学生命科学学院，北京 100048；3 东北农业大学生命科学学院，哈尔滨 150030；4 总后勤部军需装备研究所军用汉麻材料研究中心，北京 100082

出版日期:2014-11-29 发布日期:2014-12-18

Anti-UV Efficacy and Safety Evaluation of Extracts from Cannabis sativa L.

HE Cong-fen1*, LI Jing-yu2, WANG Ling3, HE Jin-feng4

1 Beijing key Laboratory of Plant Resources and Development,Beijing Technology and Business University; 2 Capital Normal University,School of Life Sciences,Beijing 100048, China; 3 Northeast Agricultural University, School of Life Science,Harbin 150030,China; 4 The Research Center of China Hemp Materials of the Quartermaster Research Institute of the General Logistic Department of the CPLA,Beijing 100082,China

Online:2014-11-29 Published:2014-12-18

摘要/Abstract

摘要： 通过紫外线光谱扫描、清除自由基试验和光溶血试验等方法对不同生长期、不同部位的汉麻提取物进行了抗紫外评价和比较，优选出的提取部位为：汉麻（160 d）叶的95%乙醇提取物，并进一步确定其最佳提取工艺条件为：料液比1:20，提取温度75 ℃，提取时间2 h，提取1次。汉麻叶提取物的主要成分为黄酮类物质（0.571 g/g）。在安全性评价中，红细胞溶血试验和鸡胚绒毛尿囊膜试验的结果均表明该汉麻叶提取物无明显刺激性。此外，该提取物还具有良好的的pH、冷、热和光稳定性，可作为一种抗紫外的植物功效原料应用于防晒化妆品的开发。

关键词: 汉麻, 抗紫外功效, 清除自由基, 光溶血实验, 安全性评价

Abstract: The anti-UV efficacy and safety of extracts of Cannabis sativa L.(China-hemp) of different growth stages and/or different parts were evaluated by means of ultraviolet spectral scanning,free radical scavenging tests and light hemolysis experiments.The screened out extract with the highest anti-UV efficacy was the 95% ethanol extract of hemp leaves (160 days).The optimized extraction parameters were determined as follows:solidliquid ratio 1:20,extraction temperature 75 ℃,extraction time 2 h,and extract for one time.The main component of the extract was flavonoids (0.571 g/g).In the safety evaluation,the results of red blood cell hemolysis test and chorioallantioc membrane experiment showed that the hemp leaf extract was not irritative.In addition,the hemp extract also had a good stability in solution with changing pH,cold,heat and light.The 95% ethanol extract of hemp leaves (160 days) had the potential to be used as a functional plant additive for anti-UV cosmetics.

Key words: China-hemp, ultraviolet, free radical-scavenging, light hemolysis test, safety evaluation

中图分类号:

Q949.97

何聪芬1*，李靖宇2，王领3，何锦风4. 汉麻提取物的抗紫外功效筛选和安全性评价[J]. 天然产物研究与开发, 2014, 26(11): 1872-1878.

HE Cong-fen1*, LI Jing-yu2, WANG Ling3, HE Jin-feng4. Anti-UV Efficacy and Safety Evaluation of Extracts from Cannabis sativa L.[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(11): 1872-1878.

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