关键词: 层孔菌属, 活性物质, 抗氧化, 相关性

Abstract: The objectives of this study were to analyze the active antioxidant substances,to determine the antioxidant activity of liquid fermentation products from Phellinus and to further investigate the correlative relationships between contents of active antioxidant components,between results from different antioxidant assays and between contents of different active antioxidant components and results from different antioxidant assays.The results showed that the contents of active antioxidant substances of different strains were obviously different,where the contents of polysaccharide and total phenol of stain H2 were the highest,and the contents of flavonoids of strain S was the highest.The correlative relationship between active substances was low.The antioxidant capacity assay showed that strain H2 had a high reducing power and Fe ion chelating ability,strain H2 and H9 had the strongest effect on hydroxyl radical scavenging,strain H2 and L had the strongest DPPH scavenging effect,and strain H2,H5,H8,H9 had the highest superoxide radical scavenging effect.In addition,there were significant differences between results from different antioxidant assay methods,in which hydroxyl radicals and DPPH free radical scavenging assays,hydroxyl radicals and Fe chelating ability assays,hydroxyl radicals and reducing power assays,DPPH and Fe chelating ability assays,DPPH and reducing power assays as well as Fe chelating ability and reducing power assays had the highest correlative relationships.There was the highest correlation between the contents of total phenolics and results from different antioxidant assays and the correlative relationships between the contents of flavonoids and results from different antioxidant assay was the second,but the correlative relationships between the contents of polysaccharide and the results from antioxidant assays were low.The above results suggested that polyphenols and flavonoids were important antioxidant factors,and had an important contribution to the antioxidant capacity.

Key words: Phellinus, active substance, antioxidant capacity, correlation